backgroundCorrect2 {OLIN} | R Documentation |
Background correction based on
backgroundCorrect
of the limma
package.
backgroundCorrect2(object,method="subtract", offset=0)
object |
object of class |
method |
method for background correction: “none”, “subtract”, “half”, “minimum”, “movingmin”, “edwards” or “normexp”. |
offset |
numeric value to add to intensities |
This function is a wrapper function for
backgroundCorrect
with following methods implemented:
“none”: no background correction
“subtract”: simple subtraction of background intensities
“movingmin”: background intensities are first averaged over 3x3 grids of neighbouring spots and subsequently substracted
“minimum”: zero or negative intensities after background correction are set equal to half the minimum of positive corrected intensities
“edwards”: background correction based on log-linear interpolation
“normexp”: background correction based on fitting procedure
For further details and references, please refer to its help page. An
alternative Bayesian model for background correction
(kooperberg
) is also implemented
in the limma
package.
Background correct object of class marrayRaw
.
Matthias Futschik
# Loading data data(sw) #No background correction sw.none <- backgroundCorrect2(sw,method="none") plot(maA(sw.none)[,1],maM(sw.none)[,1]) # Simple subtraction sw.sub <- backgroundCorrect2(sw,method="sub") points(maA(sw.sub)[,1],maM(sw.sub)[,1],col="red")