windowPerGroup {ORFik} | R Documentation |
If downstream is 20, it means the window will start 20 downstream of gr start site (-20 in relative transcript coordinates.) If upstream is 20, it means the window will start 20 upstream of gr start site (+20 in relative transcript coordinates.) It will keep exon structure of tx, so if -20 is on next exon, the previous exon is of course deleted.
windowPerGroup(gr, tx, upstream = 0L, downstream = 0L)
gr |
a GRanges object (startSites and others, must be single point) |
tx |
a GRangesList of transcripts or (container region), names of tx must contain all gr names. The names of gr can also be the ORFik orf names. that is "txName_id" |
upstream |
an integer (0), relative region to get upstream from. |
downstream |
an integer (0), relative region to get downstream from |
If a region has a part that goes out of bounds, E.g if you try to get window
around the CDS start site, goes longer than the 5' leader start site,
it will set start to the edge boundary
(the TSS of the transcript in this case).
If region has no hit in bound, a width 0 GRanges object is returned.
This is usefull for things like countOverlaps, since 0 hits will then always
be returned for the correct object. If you don't want the 0 width windows,
use reduce()
a GRanges, or GRangesList object if any group had > 1 exon.
Other ExtendGenomicRanges: asTX
,
coveragePerTiling
,
overlapsToCoverage
,
reduceKeepAttr
, tile1
,
txSeqsFromFa
# find 2nd codon of an ORF on a spliced transcript ORF <- GRanges("1", c(3), "+") # start site names(ORF) <- "tx1_1" # ORF 1 on tx1 tx <- GRangesList(tx1 = GRanges("1", c(1,3,5,7,9,11,13), "+")) windowPerGroup(ORF, tx, upstream = -3, downstream = 5) # <- 2nd codon