ctlSeparatn {phenoDist} | R Documentation

## Calculate control separation

### Description

This function calculates the Z'-factor between negative and positive controls, based on phenotypic distance measurements.

### Usage

    ctlSeparatn(x, pheno, neg='rluc', pos='ubc', ...)

### Arguments

`x` | An `imageHTS` object.
`pheno` | A numeric vector for distances to negative controls, as returned from `distToNeg`.
`neg` | A character string to identify negative controls.
`pos` | A character string to identify positive controls.
`...` | Additional arguments to be passed to the `Zprime` function of the `imageHTS` package.

### Details

Phenotypes of negative and positive controls are defined as their phenotypic distance to negative controls. The Z'-factor is a metric measuring the separation between negative controls and positive controls (Zhang et al. 1999). This function calls the `Zprime` function of the `imageHTS` package to calculate the Z'-factor. Please see `Zprime` for detailed description of the definition and calculation of the Z'-factor.

### Value

The Z'-factor value.

### Author(s)

Xian Zhang

### References

J. H. Zhang, T. D. Chung, K. R. Oldenburg. A Simple Statistical Parameter for Use in Evaluation and Validation of High Throughput Screening Assays. J Biomol Screening, 1999.

### See Also

`distToNeg`, `Zprime`

### Examples

    ## see distToNeg.

[Package phenoDist version 1.27.0 Index]