%\VignetteIndexEntry{3. A quick introduction to GRanges and GRangesList objects (slides)}
%\VignetteDepends{IRanges, GenomicRanges, pasillaBamSubset, GenomicAlignments, TxDb.Dmelanogaster.UCSC.dm3.ensGene}

\SweaveOpts{keep.source=TRUE, eps=FALSE, width=9, height=3}

\documentclass[8pt]{beamer}

\usepackage{slides}
\renewcommand\Rclass[1]{{\texttt{#1}\index{#1 (class)}}}

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\title{A quick introduction to GRanges and GRangesList objects}

\author{Herv\'e Pag\`es\\
        \href{mailto:hpages@fredhutch.org}{hpages@fredhutch.org}\\
        \---\\
        Michael Lawrence\\
        \href{mailto:lawrence.michael@gene.com}{lawrence.michael@gene.com}}

%\institute[FHCRC]{Fred Hutchinson Cancer Research Center\\
%                  Seattle, WA}

\date{July 2015}

\begin{document}

<<setup,echo=FALSE>>=
options(width=84)
plotRanges <- function(x, xlim = x, main = deparse(substitute(x)),
                       col = "black", sep = 0.5, ...)
{
  height <- 1
  if (is(xlim, "Ranges"))
    xlim <- c(min(start(xlim)), max(end(xlim)))
  bins <- disjointBins(IRanges(start(x), end(x) + 1))
  plot.new()
  par(mai=c(0.5, 0.2, 1.2, 0.2))
  plot.window(xlim, c(0, max(bins)*(height + sep)))
  ybottom <- bins * (sep + height) - height
  rect(start(x)-0.5, ybottom, end(x)+0.5, ybottom + height, col = col, ...)
  title(main, cex.main=2.8, font.main=1)
  axis(1)
}
@

\maketitle

\frame{\tableofcontents}



%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%
%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%
\section{\Rclass{GRanges} objects}
%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%
%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%


\begin{frame}[fragile]
  \frametitle{The \Rclass{GRanges} class is a container for...}
  ... storing a set of {\em genomic ranges} (a.k.a. {\em genomic regions}
  or {\em genomic intervals}).

  \begin{block}{}
    \begin{itemize}
      \item Each genomic range is described by a chromosome name,
            a {\em start}, an {\em end}, and a strand.
      \item {\em start} and {\em end} are both {\bf 1-based} positions
            relative to the 5' end of the plus strand of the chromosome,
            even when the range is on the minus strand.
      \item {\em start} and {\em end} are both considered to be included
            in the interval (except when the range is empty).
      \item The {\em width} of the range is the number of genomic positions
            included in it. So {\em width} = {\em end} - {\em start} + 1.
      \item {\em end} is always >= {\em start}, except for empty ranges
            (a.k.a. zero-width ranges) where {\em end} = {\em start} - 1.
    \end{itemize}

    Note that the {\em start} is always the leftmost position and the
    {\em end} the rightmost, even when the range is on the minus strand.
 
    Gotcha: A TSS is at the {\em end} of the range associated with a
    transcript located on the minus strand.
  \end{block}
\end{frame}


%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%
\subsection{The \Rcode{GRanges()} constructor}

\begin{frame}[fragile]
  \frametitle{The \Rcode{GRanges()} constructor}
  \begin{exampleblock}{}
{\small
<<GRanges_constructor>>=
library(GenomicRanges)
gr1 <- GRanges(seqnames=Rle(c("ch1", "chMT"), c(2, 4)),
               ranges=IRanges(16:21, 20),
               strand=rep(c("+", "-", "*"), 2))
gr1
@
}
  \end{exampleblock}
\end{frame}


%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%
\subsection{\Rclass{GRanges} accessors}

\begin{frame}[fragile]
  \frametitle{\Rclass{GRanges} accessors: \Rcode{length()},
              \Rcode{seqnames()}, \Rcode{ranges()}}

  \begin{exampleblock}{}
{\small
<<GRanges_accessors1>>=
length(gr1)
seqnames(gr1)
ranges(gr1)
@
}
  \end{exampleblock}
\end{frame}

\begin{frame}[fragile]
  \frametitle{\Rclass{GRanges} accessors: \Rcode{start()}, \Rcode{end()},
              \Rcode{width()}, \Rcode{strand()}}

  \begin{exampleblock}{}
{\small
<<GRanges_accessors2>>=
start(gr1)
end(gr1)
width(gr1)
strand(gr1)
strand(gr1) <- c("-", "-", "+")
strand(gr1)
@
}
  \end{exampleblock}
\end{frame}

\begin{frame}[fragile]
  \frametitle{\Rclass{GRanges} accessors: \Rcode{names()}}

  \begin{exampleblock}{}
{\small
<<GRanges_accessors3>>=
names(gr1) <- LETTERS[1:6]
gr1
names(gr1)
@
}
  \end{exampleblock}
\end{frame}

\begin{frame}[fragile]
  \frametitle{\Rclass{GRanges} accessors: \Rcode{mcols()}}

  Like with most \Bioconductor{} vector-like objects, {\em metadata columns}
  can be added to a \Rclass{GRanges} object:
  \begin{exampleblock}{}
{\small
<<GRanges_accessors4>>=
mcols(gr1) <- DataFrame(score=11:16, GC=seq(1, 0, length=6))
gr1
mcols(gr1)
@
}
  \end{exampleblock}
\end{frame}

\begin{frame}[fragile]
  \frametitle{\Rclass{GRanges} accessors: \Rcode{seqinfo()},
              \Rcode{seqlevels()}, \Rcode{seqlengths()}}

  \begin{exampleblock}{}
<<GRanges_accessors5>>=
seqinfo(gr1)
seqlevels(gr1)
seqlengths(gr1)
seqlengths(gr1) <- c(50000, 800)
seqlengths(gr1)
@
  \end{exampleblock}
\end{frame}


%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%
\subsection{Vector operations on \Rclass{GRanges} objects}

\setbeamertemplate{background canvas}[vertical shading][bottom=green!20,top=green!20]
\begin{frame}[fragile]
  \frametitle{Vector operations on \Rclass{GRanges} objects}
  \begin{block}{}
    What we call {\em vector operations} are operations that work on any
    ordinary vector:

    \begin{itemize}
      \item \Rcode{length()}, \Rcode{names()}
      \item Single-bracket subsetting: \Rcode{[}
      \item Combining: \Rcode{c()}
      \item \Rcode{split()}, \Rcode{relist()}
      \item Comparing: \Rcode{==}, \Rcode{!=}, \Rcode{match()}, \Rcode{\%in\%},
                       \Rcode{duplicated()}, \Rcode{unique()}
      \item Ordering: \Rcode{<=}, \Rcode{>=}, \Rcode{<}, \Rcode{>},
                      \Rcode{order()}, \Rcode{sort()}, \Rcode{rank()}
    \end{itemize}

    \Rclass{GRanges} objects support all these {\em vector operations}
    $==>$ They're considered {\em vector-like} objects.
  \end{block}
\end{frame}
\DefaultBackground

\begin{frame}[fragile]
  \frametitle{Vector operations on \Rclass{GRanges} objects:
              Single-bracket subsetting}
  \begin{exampleblock}{}
{\small
<<GRanges_Vector_ops1>>=
gr1[c("F", "A")]
gr1[strand(gr1) == "+"]
@
}
  \end{exampleblock}
\end{frame}

\begin{frame}[fragile]
  \frametitle{Vector operations on \Rclass{GRanges} objects:
              Single-bracket subsetting}
  \begin{exampleblock}{}
{\small
<<GRanges_Vector_ops2>>=
gr1 <- gr1[-5]
gr1
@
}
  \end{exampleblock}
\end{frame}

\begin{frame}[fragile]
  \frametitle{Vector operations on \Rclass{GRanges} objects: Combining}
  \begin{exampleblock}{}
{\small
<<GRanges_Vector_ops3>>=
gr2 <- GRanges(seqnames="ch2",
               ranges=IRanges(start=c(2:1,2), width=6),
               score=15:13,
               GC=seq(0, 0.4, length=3))
gr12 <- c(gr1, gr2)
gr12
@
}
  \end{exampleblock}
\end{frame}

\begin{frame}[fragile]
  \frametitle{Vector operations on \Rclass{GRanges} objects: Comparing}
  \begin{exampleblock}{}
{\small
<<GRanges_Vector_ops4>>=
gr12[length(gr12)] == gr12
duplicated(gr12)
unique(gr12)
@
}
  \end{exampleblock}
\end{frame}

\begin{frame}[fragile]
  \frametitle{Vector operations on \Rclass{GRanges} objects: Ordering}
  \begin{exampleblock}{}
{\small
<<GRanges_sort>>=
sort(gr12)
@
}
  \end{exampleblock}
\end{frame}

\begin{frame}[fragile]
  \frametitle{Splitting a \Rclass{GRanges} object}
  \begin{exampleblock}{}
{\small
<<GRanges_split>>=
split(gr12, seqnames(gr12))
@
}
  \end{exampleblock}
\end{frame}

\setbeamertemplate{background canvas}[vertical shading][bottom=orange!40,top=orange!20]
\begin{frame}[fragile]
  \frametitle{Exercise 1}
  \setbeamertemplate{enumerate items}{\alph{enumi}.}
  \begin{enumerate}
    \item Load the \Rpackage{GenomicRanges} package.
    \item Open the man page for the \Rclass{GRanges} class and run the
          examples in it.
    \item Extract from \Rclass{GRanges} object \Rcode{gr} the elements (i.e.
          ranges) with a score between 4 and 8.
    \item Split \Rcode{gr} by strand.
  \end{enumerate}
\end{frame}
\DefaultBackground


%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%
\subsection{Range-based operations on \Rclass{GRanges} objects}

\setbeamertemplate{background canvas}[vertical shading][bottom=green!20,top=green!20]
\begin{frame}[fragile]
  \frametitle{An overview of {\em range-based} operations}

  \begin{columns}[t]
    \begin{column}{0.44\textwidth}
      \begin{block}{}
        {\bf Intra range transformations}

        \Rcode{shift()}, \Rcode{narrow()}, \Rcode{resize()}, \Rcode{flank()}
      \end{block}
      \begin{block}{}
        {\bf Inter range transformations}

        \Rcode{range()}, \Rcode{reduce()}, \Rcode{gaps()}, \Rcode{disjoin()}
      \end{block}
      \begin{block}{}
        {\bf Range-based set operations}

        \Rcode{union()}, \Rcode{intersect()}, \Rcode{setdiff()},
        \Rcode{punion()}, \Rcode{pintersect()}, \Rcode{psetdiff()},
        \Rcode{pgap()}
      \end{block}
    \end{column}
    \begin{column}{0.44\textwidth}
      \begin{block}{}
        {\bf Coverage and slicing}

        \Rcode{coverage()}, \Rcode{slice()}
      \end{block}
      \begin{block}{}
        {\bf Finding/counting overlapping ranges}

        \Rcode{findOverlaps()}, \Rcode{countOverlaps()}
      \end{block}
      \begin{block}{}
        {\bf Finding the nearest range neighbor}

        \Rcode{nearest()}, \Rcode{precede()}, \Rcode{follow()}
      \end{block}
      \begin{block}{}
        and more...
      \end{block}
    \end{column}
  \end{columns}
\end{frame}

\begin{frame}[fragile]
  \frametitle{Examples of some common {\em range-based} operations}
  \begin{exampleblock}{}
<<ranges-ir0-plot,results=hide,echo=FALSE,fig=FALSE>>=
library(IRanges)
ir0 <- IRanges(start=c(7, 9, 12, 14, 22:24),
               end=c(15, 11, 12, 18, 26, 27, 28))

png("ranges-ir0-plot.png", width=800, height=170)
plotRanges(ir0, xlim=c(5, 35), main="ir0", col="blue")
dev.off()
@
<<ranges-shift-ir0-plot,results=hide,echo=FALSE,fig=FALSE>>=
png("ranges-shift-ir0-plot.png", width=800, height=170)
plotRanges(shift(ir0, 5), xlim=c(5, 35), main="shift(ir0, 5)", col="blue")
dev.off()
@
<<ranges-reduce-ir0-plot,results=hide,echo=FALSE,fig=FALSE>>=
png("ranges-reduce-ir0-plot.png", width=800, height=170)
plotRanges(reduce(ir0), xlim=c(5, 35), main="reduce(ir0)", col="blue")
dev.off()
@
<<ranges-disjoin-ir0-plot,results=hide,echo=FALSE,fig=FALSE>>=
png("ranges-disjoin-ir0-plot.png", width=800, height=170)
plotRanges(disjoin(ir0), xlim=c(5, 35), main="disjoin(ir0)", col="blue")
dev.off()
@
    \begin{figure}
      \centering
      \includegraphics[width=0.8\textwidth,height=!]{ranges-ir0-plot}\\
      \includegraphics[width=0.8\textwidth,height=!]{ranges-shift-ir0-plot}\\
      \includegraphics[width=0.8\textwidth,height=!]{ranges-reduce-ir0-plot}\\
      \includegraphics[width=0.8\textwidth,height=!]{ranges-disjoin-ir0-plot}
      %\caption{Range-based operations}
    \end{figure}
  \end{exampleblock}
\end{frame}
\DefaultBackground

\begin{frame}[fragile]
  \frametitle{Range-based operations on \Rclass{GRanges} objects}
  \begin{exampleblock}{}
{\small
<<GRanges_Ranges_ops1>>=
gr2
shift(gr2, 50)
@
}
  \end{exampleblock}
\end{frame}

\begin{frame}[fragile]
  \frametitle{Range-based operations on \Rclass{GRanges} objects (continued)}
  \begin{exampleblock}{}
{\small
<<GRanges_Ranges_ops2>>=
gr1
resize(gr1, 12)
@
}
  \end{exampleblock}
\end{frame}

\begin{frame}[fragile]
  \frametitle{Range-based operations on \Rclass{GRanges} objects (continued)}
  \begin{exampleblock}{}
{\small
<<GRanges_Ranges_ops3>>=
gr1
flank(gr1, 3)
@
}
  \end{exampleblock}
\end{frame}

\begin{frame}[fragile]
  \frametitle{Range-based operations on \Rclass{GRanges} objects (continued)}
  \begin{exampleblock}{}
{\small
<<GRanges_range>>=
gr3 <- shift(gr1, c(35000, rep(0, 3), 100))
width(gr3)[c(3,5)] <- 117
gr3
range(gr3)
@
}
  \end{exampleblock}
\end{frame}

\begin{frame}[fragile]
  \frametitle{Range-based operations on \Rclass{GRanges} objects (continued)}
  \begin{exampleblock}{}
{\small
<<GRanges_reduce>>=
gr3
reduce(gr3)
@
}
  \end{exampleblock}
\end{frame}

\begin{frame}[fragile]
  \frametitle{Range-based operations on \Rclass{GRanges} objects (continued)}
  \begin{exampleblock}{}
{\scriptsize
<<GRanges_gaps>>=
gr3
gaps(gr3)
@
}
  \end{exampleblock}
\end{frame}

\begin{frame}[fragile]
  \frametitle{Range-based operations on \Rclass{GRanges} objects (continued)}
  \begin{exampleblock}{}
{\scriptsize
<<GRanges_disjoin>>=
gr3
disjoin(gr3)
@
}
  \end{exampleblock}
\end{frame}

\setbeamertemplate{background canvas}[vertical shading][bottom=orange!40,top=orange!20]
\begin{frame}[fragile]
  \frametitle{Exercise 2}
  \setbeamertemplate{enumerate items}{\alph{enumi}.}
  Using \Rclass{GRanges} object \Rcode{gr} created at Exercise 1:
  \begin{enumerate}
    \item Shift the ranges in \Rcode{gr} by 1000 positions to the right.
    \item What method is called when doing \Rcode{shift()} on a
          \Rclass{GRanges} object? Find the man page for this method.
  \end{enumerate}
\end{frame}
\DefaultBackground

\begin{frame}[fragile]
  \frametitle{Coverage}
  \begin{exampleblock}{}
{\small
<<GRanges_coverage1>>=
cvg12 <- coverage(gr12)
cvg12
@
}
  \end{exampleblock}
\end{frame}

\begin{frame}[fragile]
  \frametitle{Coverage (continued)}
  \begin{exampleblock}{}
{\small
<<GRanges_coverage2>>=
mean(cvg12)
max(cvg12)
@
}
  \end{exampleblock}
\end{frame}

\begin{frame}[fragile]
  \frametitle{Slicing the coverage}
  \begin{exampleblock}{}
{\small
<<slice_coverage>>=
sl12 <- slice(cvg12, lower=1)
sl12
elementNROWS(sl12)
sl12$chMT
mean(sl12$chMT)
max(sl12$chMT)
@
}
  \end{exampleblock}
\end{frame}

\begin{frame}[fragile]
  \frametitle{findOverlaps()}

  \begin{block}{}
  Load aligned reads from a BAM file:
  \end{block}
  \begin{exampleblock}{}
{\small
<<findOverlaps1>>=
library(pasillaBamSubset)
untreated1_chr4()
library(GenomicAlignments)
reads <- readGAlignments(untreated1_chr4())
@
}
  \end{exampleblock}

  \begin{block}{}
  and store them in a \Rclass{GRanges} object:
  \end{block}
  \begin{exampleblock}{}
{\small
<<findOverlaps2>>=
reads <- as(reads, "GRanges")
reads[1:4]
@
}
  \end{exampleblock}
\end{frame}

\begin{frame}[fragile]
  \frametitle{findOverlaps() (continued)}

  \begin{block}{}
  Load the gene ranges from a {\em TxDb} package: 
  \end{block}
  \begin{exampleblock}{}
{\small
<<findOverlaps3>>=
library(TxDb.Dmelanogaster.UCSC.dm3.ensGene)
txdb <- TxDb.Dmelanogaster.UCSC.dm3.ensGene
dm3_genes <- genes(txdb)
@
}
  \end{exampleblock}

  \begin{block}{}
  and find the overlaps between the reads and the genes:
  \end{block}
  \begin{exampleblock}{}
{\small
<<findOverlaps4>>=
hits <- findOverlaps(reads, dm3_genes)
head(hits)
@
}
  \end{exampleblock}
\end{frame}

\setbeamertemplate{background canvas}[vertical shading][bottom=orange!40,top=orange!20]
\begin{frame}[fragile]
  \frametitle{Exercise 3}
  \setbeamertemplate{enumerate items}{\alph{enumi}.}
  \begin{enumerate}
    \item Recreate \Rclass{GRanges} objects \Rcode{reads} and
          \Rcode{dm3\_genes} from previous slides.
    \item What method is called when calling \Rcode{findOverlaps()} on
          them? Open the man page for this method.
    \item Find the overlaps between the 2 objects but this time the strand
          should be ignored.
  \end{enumerate}
\end{frame}
\DefaultBackground

\setbeamertemplate{background canvas}[vertical shading][bottom=orange!40,top=orange!20]
\begin{frame}[fragile]
  \frametitle{Exercise 4}
  \setbeamertemplate{enumerate items}{\alph{enumi}.}
  In this exercise we want to get the exon sequences for the dm3 genome.
  \begin{enumerate}
    \item Extract the exon ranges from \Rcode{txdb}.
    \item Load the \Rpackage{BSgenome.Dmelanogaster.UCSC.dm3} package.
    \item Use \Rcode{getSeq()} to extract the exon sequences from the
          \Rcode{BSgenome} object in \Rpackage{BSgenome.Dmelanogaster.UCSC.dm3}.
  \end{enumerate}
\end{frame}
\DefaultBackground


%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%
%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%
\section{\Rclass{GRangesList} objects}
%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%
%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%


\begin{frame}[fragile]
  \frametitle{The \Rclass{GRangesList} class is a container for...}
  storing a list of {\em compatible} \Rclass{GRanges} objects.

  \begin{block}{}
    {\em compatible} means:
    \begin{itemize}
      \item they are relative to the same genome,
      \item AND they have the same metadata columns (accessible with
            the \Rcode{mcols()} accessor).
    \end{itemize}
  \end{block}
\end{frame}


%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%
\subsection{The \Rcode{GRangesList()} constructor}

\begin{frame}[fragile]
  \frametitle{The \Rcode{GRangesList()} constructor}
  \begin{exampleblock}{}
{\small
<<GRangesList_constructor>>=
grl <- GRangesList(gr3, gr2)
grl
@
}
  \end{exampleblock}
\end{frame}


%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%
\subsection{\Rclass{GRangesList} accessors}

\begin{frame}[fragile]
  \frametitle{\Rclass{GRangesList} accessors}
  \begin{exampleblock}{}
<<GRangesList_accessors1>>=
length(grl)
@
  \end{exampleblock}
  \begin{columns}[t]
    \begin{column}{0.44\textwidth}
      \begin{exampleblock}{}
{\small
<<GRangesList_accessors2>>=
seqnames(grl)
@
}
      \end{exampleblock}
    \end{column}
    \begin{column}{0.44\textwidth}
      \begin{exampleblock}{}
{\small
<<GRangesList_accessors3>>=
strand(grl)
@
}
      \end{exampleblock}
    \end{column}
  \end{columns}
\end{frame}

\begin{frame}[fragile]
  \frametitle{\Rclass{GRangesList} accessors (continued)}
  \begin{columns}[t]
    \begin{column}{0.44\textwidth}
      \begin{exampleblock}{}
{\small
<<GRangesList_accessors4>>=
ranges(grl)
@
}
      \end{exampleblock}
    \end{column}
    \begin{column}{0.44\textwidth}
      \begin{exampleblock}{}
{\small
<<GRangesList_accessors5>>=
start(grl)
end(grl)
width(grl)
@
}
      \end{exampleblock}
    \end{column}
  \end{columns}
\end{frame}

\begin{frame}[fragile]
  \frametitle{\Rclass{GRangesList} accessors (continued)}
  \begin{exampleblock}{}
{\small
<<GRangesList_accessors6>>=
names(grl) <- c("TX1", "TX2")
grl
@
}
  \end{exampleblock}
\end{frame}

\begin{frame}[fragile]
  \frametitle{\Rclass{GRangesList} accessors (continued)}
  \begin{exampleblock}{}
{\scriptsize
<<GRangesList_accessors7>>=
mcols(grl)$geneid <- c("GENE1", "GENE2") 
mcols(grl)
grl
@
}
  \end{exampleblock}
\end{frame}

\begin{frame}[fragile]
  \frametitle{\Rclass{GRangesList} accessors (continued)}
  \begin{exampleblock}{}
<<GRangesList_accessors8>>=
seqinfo(grl)
@
  \end{exampleblock}
\end{frame}


%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%
\subsection{Vector operations on \Rclass{GRangesList} objects}

\setbeamertemplate{background canvas}[vertical shading][bottom=green!20,top=green!20]
\begin{frame}[fragile]
  \frametitle{Vector operations on \Rclass{GRangesList} objects}
  \begin{block}{}
  Only the following {\em vector operations} are supported on
  \Rclass{GRangesList} objects:
    \begin{itemize}
      \item \Rcode{length()}, \Rcode{names()}
      \item Single-bracket subsetting: \Rcode{[}
      \item Combining: \Rcode{c()}
    \end{itemize}
  \end{block}
\end{frame}
\DefaultBackground

\begin{frame}[fragile]
  \frametitle{Vector operations on \Rclass{GRangesList} objects}
  \begin{exampleblock}{}
{\small
<<GRangesList_Vector_ops1>>=
grl[c("TX2", "TX1")]
@
}
  \end{exampleblock}
\end{frame}

\begin{frame}[fragile]
  \frametitle{Vector operations on \Rclass{GRangesList} objects (continued)}
  \begin{exampleblock}{}
{\scriptsize
<<GRangesList_Vector_ops2>>=
c(grl, GRangesList(gr3))
@
}
  \end{exampleblock}
\end{frame}


%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%
\subsection{List operations on \Rclass{GRangesList} objects}

\setbeamertemplate{background canvas}[vertical shading][bottom=green!20,top=green!20]
\begin{frame}[fragile]
  \frametitle{List operations on \Rclass{GRangesList} objects}
  \begin{block}{}
    What we call {\em list operations} are operations that work on an
    ordinary list:
    \begin{itemize}
      \item Double-bracket subsetting: \Rcode{[[}
      \item \Rcode{elementNROWS()}, \Rcode{unlist()}
      \item \Rcode{lapply()}, \Rcode{sapply()}, \Rcode{endoapply()}
      \item \Rcode{mendoapply()} (not covered in this presentation)
    \end{itemize}

    \Rclass{GRangesList} objects support all these {\em list operations}
    $==>$ They're considered {\em list-like} objects.
  \end{block}
\end{frame}
\DefaultBackground

\begin{frame}[fragile]
  \frametitle{elementNROWS() and unlist()}
  \begin{exampleblock}{}
{\scriptsize
<<GRangesList_List_ops1>>=
grl[[2]]
elementNROWS(grl)
unlisted <- unlist(grl, use.names=FALSE)  # same as c(grl[[1]], grl[[2]])
unlisted
@
}
  \end{exampleblock}
\end{frame}

\begin{frame}[fragile]
  \frametitle{relist()}
  \begin{exampleblock}{}
{\small
<<GRangesList_List_ops2>>=
grl100 <- relist(shift(unlisted, 100), grl)
grl100
@
}
  \end{exampleblock}
\end{frame}

\begin{frame}[fragile]
  \frametitle{endoapply()}
  \begin{exampleblock}{}
{\scriptsize
<<GRangesList_List_ops3>>=
grl100b <- endoapply(grl, shift, 100)
grl100b
mcols(grl100)
mcols(grl100b)
@
}
  \end{exampleblock}
\end{frame}


%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%
\subsection{Range-based operations on \Rclass{GRangesList} objects}

\begin{frame}[fragile]
  \frametitle{Range-based operations on \Rclass{GRangesList} objects}
  \begin{columns}[t]
    \begin{column}{0.49\textwidth}
      \begin{exampleblock}{}
{\scriptsize
<<GRangesList_Ranges_ops1>>=
grl
@
}
      \end{exampleblock}
    \end{column}
    \begin{column}{0.49\textwidth}
      \begin{exampleblock}{}
{\scriptsize
<<GRangesList_Ranges_ops2>>=
shift(grl, 100)
@
}
      \end{exampleblock}
    \end{column}
  \end{columns}
  \begin{block}{}
    \Rcode{shift(grl, 100)} is equivalent to \Rcode{endoapply(grl, shift, 100)}
  \end{block}
\end{frame}

\begin{frame}[fragile]
  \frametitle{Range-based operations on \Rclass{GRangesList}
              objects (continued)}
  \begin{columns}[t]
    \begin{column}{0.49\textwidth}
      \begin{exampleblock}{}
{\scriptsize
<<GRangesList_Ranges_ops3>>=
grl
@
}
      \end{exampleblock}
    \end{column}
    \begin{column}{0.49\textwidth}
      \begin{exampleblock}{}
{\scriptsize
<<GRangesList_Ranges_ops4>>=
flank(grl, 10)
@
}
      \end{exampleblock}
    \end{column}
  \end{columns}
  \begin{block}{}
    \Rcode{flank(grl, 10)} is equivalent to \Rcode{endoapply(grl, flank, 10)}
  \end{block}
\end{frame}

\begin{frame}[fragile]
  \frametitle{Range-based operations on \Rclass{GRangesList}
              objects (continued)}
  \begin{columns}[t]
    \begin{column}{0.49\textwidth}
      \begin{exampleblock}{}
{\scriptsize
<<GRangesList_Ranges_ops5>>=
grl
@
}
      \end{exampleblock}
    \end{column}
    \begin{column}{0.49\textwidth}
      \begin{exampleblock}{}
{\scriptsize
<<GRangesList_Ranges_ops6>>=
range(grl) 
@
}
      \end{exampleblock}
    \end{column}
  \end{columns}
  \begin{block}{}
    \Rcode{range(grl)} is equivalent to \Rcode{endoapply(grl, range)}
  \end{block}
\end{frame}

\begin{frame}[fragile]
  \frametitle{Range-based operations on \Rclass{GRangesList}
              objects (continued)}
  \begin{columns}[t]
    \begin{column}{0.49\textwidth}
      \begin{exampleblock}{}
{\scriptsize
<<GRangesList_Ranges_ops7>>=
grl
@
}
      \end{exampleblock}
    \end{column}
    \begin{column}{0.49\textwidth}
      \begin{exampleblock}{}
{\scriptsize
<<GRangesList_Ranges_ops8>>=
reduce(grl) 
@
}
      \end{exampleblock}
    \end{column}
  \end{columns}
  \begin{block}{}
    \Rcode{reduce(grl)} is equivalent to \Rcode{endoapply(grl, reduce)}
  \end{block}
\end{frame}

\begin{frame}[fragile]
  \frametitle{Range-based operations on \Rclass{GRangesList}
              objects (continued)}
<<GRangesList_Ranges_ops9,results=hide,echo=FALSE>>=
grl2 <- grl
grl2[[1]] <- grl2[[1]][3]; grl2[[2]] <- grl2[[2]][1]
grl3 <- unname(grl2)
grl3[[1]] <- narrow(unname(grl3[[1]]), start=5, end=-5)
@
  \begin{columns}[t]
    \begin{column}{0.49\textwidth}
      \begin{exampleblock}{}
{\scriptsize
<<GRangesList_Ranges_ops10>>=
grl2
grl3
@
}
      \end{exampleblock}
    \end{column}
    \begin{column}{0.49\textwidth}
      \begin{exampleblock}{}
{\scriptsize
<<GRangesList_Ranges_ops11>>=
setdiff(grl2, grl3)
@
}
      \end{exampleblock}
    \end{column}
  \end{columns}
  \begin{block}{}
    \Rcode{setdiff(grl2, grl)} is equivalent to
    \Rcode{mendoapply(setdiff, grl2, grl)}
  \end{block}
\end{frame}



%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%
%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%
\section{Other resources}
%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%
%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%

\begin{frame}[fragile]
  \frametitle{Other resources}
    \begin{itemize}
      \item Great slides from Michael on ranges sequences and alignments:
            \url{http://bioconductor.org/help/course-materials/2014/CSAMA2014/2_Tuesday/lectures/Ranges_Sequences_and_Alignments-Lawrence.pdf}

      \item Vignettes in the \Rpackage{GenomicRanges} package
            (\Rcode{browseVignettes("GenomicRanges")}).

      \item \Rclass{GRanges} and \Rclass{GRangesList} man pages in the
            \Rpackage{GenomicRanges} package.

      \item Vignettes and \Rclass{GAlignments} man page in the
            \Rpackage{GenomicAlignments} package.

      \item \Bioconductor{} support site:
            \url{http://support.bioconductor.org/}

      \item The {\em genomic ranges} paper:
        Michael Lawrence, Wolfgang Huber, Herv\'e Pag\`es, Patrick Aboyoun,
        Marc Carlson, Robert Gentleman, Martin T. Morgan, Vincent J. Carey.
        Software for Computing and Annotating Genomic Ranges.
        {\em PLOS Computational Biology}, 4(3), 2013.
    \end{itemize}
\end{frame}

%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%

\end{document}

