compute_kmer_enrichment {transite} | R Documentation

## k-mer Enrichment between Foreground and Background Sets

### Description

Compares foreground sequence set to background sequence set and computes enrichment values for each possible k-mer.

### Usage

    compute_kmer_enrichment(
      foreground_kmers,
      background_kmers,
      permutation = FALSE,
      chisq_p_value_threshold = 0.05,
      p_adjust_method = "BH"
    )

### Arguments

`foreground_kmers` | k-mer counts of the foreground set (generated by `generate_kmers`)
`background_kmers` | k-mer counts of the background set (generated by `generate_kmers`)
`permutation` | if `TRUE`, only the enrichment value is returned (efficiency mode used for permutation testing)
`chisq_p_value_threshold` | threshold below which Fisher's exact test is used instead of Pearson's chi-squared test
`p_adjust_method` | see `p.adjust`

### Details

Usually uses Pearson's chi-squared test, but recalculates p-values with Fisher's exact test for Pearson's chi-squared test p-values `<= chisq_p_value_threshold`. The reason this is done is computational efficiency. Fisher's exact tests are computationally demanding and are only performed in situations, where exact p-values are preferred, e.g., if expected < 5 or significant p-values.

### Value

enrichment of k-mers in specified foreground sequences. A data frame with the following columns is returned:

`foreground_count` | foreground counts for each k-mer
`background_count` | background counts for each k-mer
`enrichment` | k-mer enrichment
`p_value` | p-value of k-mer enrichment (either from Fisher's exact test or Pearson's chi-squared test)
`adj_p_value` | multiple testing corrected p-value

### See Also

Other k-mer functions: `calculate_kmer_enrichment()`, `check_kmers()`, `count_homopolymer_corrected_kmers()`, `draw_volcano_plot()`, `estimate_significance_core()`, `estimate_significance()`, `generate_kmers()`, `generate_permuted_enrichments()`, `run_kmer_spma()`, `run_kmer_tsma()`

### Examples

    # define simple sequence sets for foreground and background
    foreground_set <- c(
      "CAACAGCCUUAAUU", "CAGUCAAGACUCC", "CUUUGGGGAAU",
      "UCAUUUUAUUAAA", "AAUUGGUGUCUGGAUACUUCCCUGUACAU",
      "AUCAAAUUA", "AGAU", "GACACUUAAAGAUCCU",
      "UAGCAUUAACUUAAUG", "AUGGA", "GAAGAGUGCUCA",
      "AUAGAC", "AGUUC", "CCAGUAA"
    )
    background_set <- c(
      "CAACAGCCUUAAUU", "CAGUCAAGACUCC", "CUUUGGGGAAU",
      "UCAUUUUAUUAAA", "AAUUGGUGUCUGGAUACUUCCCUGUACAU",
      "AUCAAAUUA", "AGAU", "GACACUUAAAGAUCCU",
      "UAGCAUUAACUUAAUG", "AUGGA", "GAAGAGUGCUCA",
      "AUAGAC", "AGUUC", "CCAGUAA",
      "UUAUUUA", "AUCCUUUACA", "UUUUUUU", "UUUCAUCAUU",
      "CCACACAC", "CUCAUUGGAG", "ACUUUGGGACA", "CAGGUCAGCA"
    )
    foreground_kmers <- generate_kmers(foreground_set, 6)
    background_kmers <- generate_kmers(background_set, 6)

    kmer_enrichment_values <- compute_kmer_enrichment(foreground_kmers,
      background_kmers)

[Package transite version 1.10.0 Index]