setAllelePhase {sangerseqR} | R Documentation

## Set Reference and Alternate Alleles

### Description

Parses the Primary and Secondary Sequences into Reference and Alternate Alleles

### Usage

    setAllelePhase(obj, refseq, trim5 = 0, trim3 = 0)

    ## S4 method for signature 'sangerseq'
    setAllelePhase(obj, refseq, trim5 = 0, trim3 = 0)

### Arguments

`obj` | `sangerseq` class object
`refseq` | DNAString for character string of reference allele sequence.
`trim5` | Number of bases to trim from the beginning of the sequence.
`trim3` | Number of bases to trim from the end of the sequence.

### Details

When multiple heterozygous basecalls are made, it is generally unclear which calls are in phase with each other. This function takes a reference sequence for one of the alleles to match the primary and secondary basecalls as reference or alternate allele.

### Value

A `sangerseq` object with the Reference Allele in the primarySeq slot and the Alternate Allele in the secondarySeq slot.

### See Also

`makeBaseCalls`, `chromatogram`, `sangerseq`

### Examples

    #Load Sequences
    hetsangerseq <- readsangerseq(system.file("extdata",
                                              "heterozygous.ab1",
                                              package = "sangerseqR"))
    homosangerseq <- readsangerseq(system.file("extdata",
                                               "homozygous.scf",
                                               package = "sangerseqR"))
    #Make calls on heterozygous sequence to be parsed
    hetcalls <- makeBaseCalls(hetsangerseq, ratio = 0.33)
    #Need a reference sequence to set phase. Can get from annotation
    #(e.g. Refseq) or another sanger sequencing file
    ref <- subseq(primarySeq(homosangerseq, string = TRUE),
                  start = 30,
                  width = 500)
    #Set the phase
    hetseqalleles <- setAllelePhase(hetcalls, ref, trim5 = 50, trim3 = 100)
    #Align to compare alleles
    pa <- pairwiseAlignment(primarySeq(hetseqalleles),
                                       secondarySeq(hetseqalleles),
                                       type = "global-local")
    writePairwiseAlignments(pa)

[Package sangerseqR version 1.28.0 Index]