extend_pe_to_gg {multicrispr} | R Documentation

## Extend prime editing target to find GG sites

### Description

Extend prime editing target to find GG sites in accessible neighbourhood

### Usage

    extend_pe_to_gg(gr, nrt = 16, plot = FALSE)

### Arguments

`gr` | target `GRanges-class`
`nrt` | n RT nucleotides (default 16, recommended 10-16)
`plot` | TRUE or FALSE (default)

### Details

Extends each target range to the area in which to search for a prime editing GG duplet, as shown in the sketch below.

===============> —-GG———> —-GG———> ** <———GG— <———GG—- <===============

### Value

`GRanges-class`

### Examples

    # PE example
    #-----------
        require(magrittr)
        bsgenome <- BSgenome.Hsapiens.UCSC.hg38::BSgenome.Hsapiens.UCSC.hg38
        gr <- char_to_granges(c(PRNP = 'chr20:4699600:+',             # snp
                               HBB  = 'chr11:5227002:-',              # snp
                               HEXA = 'chr15:72346580-72346583:-',    # del
                               CFTR = 'chr7:117559593-117559595:+'),  # ins
                              bsgenome)
        extend_pe_to_gg(gr, plot = TRUE)

[Package multicrispr version 1.2.0 Index]