plotBins {flowStats} | R Documentation

## Plots the probability bins overlaid with flowFrame data

### Description

This function is useful in visualizing the differences between the binned control and sample datasets. The bins generated from the control dataset are overlaid with the sample dataset. An optional argument residuals can be used to shade each bin based on a calculated statistical measure of difference between the number of events in each bin.

### Usage

    plotBins(binRes,data,channels,title,residuals,shadeFactor)

### Arguments

`binRes` | The result generated by calling the `probBin` function on a control dataset.
`data` | An object of class `flowFrame` sample(dataset)
`channels` | The flow parameters to be plotted.In cases where more than two parameters are binned from the control set, the `plotBins` function plots the projections of the hyperplanes in 2 dimensions)
`title` | Optional title for the plot generated
`residuals` | A vector of length equal to the number of bins generated that can be used to shade each bin. The residuals from the `calcPearsonChi` function or the `calcPBChiSquare` function can be used to highlight the bins that are different between control and sample datasets
`shadeFactor` | Optional argument between 0 and 1 that controls the intensity of the shading of bins

### Author(s)

Nishant Gopalakrishnan

### See Also

`proBin`, `calcPearsonChi`, `calcPBChiSquare`

### Examples

    library(flowCore)
    data(GvHD)
    # flow frame 1 is treated as  control dataset and used to generate bins
    resCtrl<-proBin(GvHD[[1]],200,channels=c("FSC-H","SSC-H"))
    plotBins(resCtrl,GvHD[[1]],channels=c("FSC-H","SSC-H"),title="Binned control data")
    # Same bins are applied to flowFrame 16
    resSample<-binByRef(resCtrl,GvHD[[16]])
    stat<-calcPearsonChi(resCtrl,resSample)
    dev.new()
    plotBins(resCtrl,data=GvHD[[16]],channels=c("FSC-H","SSC-H","Time"),title="Comparision 1 & 16",
    residuals=stat$residuals[2,],shadeFactor=0.7)

[Package flowStats version 4.4.0 Index]