VRanges-class {VariantAnnotation} | R Documentation

## VRanges objects

### Description

The VRanges class is a container for variant calls, including SNVs and indels. It extends `GRanges` to provide special semantics on top of a simple vector of genomic locations. While it is not as expressive as the `VCF` object, it is a simpler alternative that may be convenient for variant calling/filtering and similar exercises.

### Details

VRanges extends GRanges to store the following components. Except where noted, the components are considered columns in the dataset, i.e., their lengths match the number of variants. Many columns can be stored as either an atomic vector or an Rle.

`ref`

  (`character`), the reference allele. The range (start/end/width) should always correspond to this sequence.

`alt`

  (`character/Rle`), the alternative allele (NA allowed). By convention there is only a single alt allele per element (row) of the VRanges. Many methods, like `match`, make this assumption.

`refCount`

  (`integer/Rle`), read count for the reference allele (NA allowed)

`altCount`

  (`integer/Rle`), read count for the alternative allele (NA allowed)

`totalCount`

  (`integer/Rle`), total read count at the position, must be at least `refCount+altCount` (NA allowed)

`sampleNames`

  (`factor/Rle`), name of the sample - results from multiple samplse can be combined into the same object (NA allowed)

`softFilterMatrix`

  (`matrix/FilterMatrix`), variant by filter matrix, `TRUE` where variant passed the filter; use a `FilterMatrix` to store the actual `FilterRules` object that was applied

`hardFilters`

  (`FilterRules`) record of hard filters applied, i.e., only the variants that passed the filters are present in this object; this is the only component that is not a column, i.e., its length does not match the number of variants

Except in the special circumstances described here, a `VRanges` may be treated like a `GRanges`. The range should span the sequence in `ref`. Indels are typically represented by the VCF convention, i.e., the start position is one upstream of the event. The strand is always constrained to be positive (+).

Indels, by convention, should be encoded VCF-style, with the upstream reference base prepended to the indel sequence. The ref/alt for a deletion of GCGT before A might be AGCGT/A and for an insertion might be A/AGCGT. Since the range always matches the `ref` sequence, this means a deletion will be the width of the deletion + 1, and an insertion is always of width 1.

VRanges and the VCF class: The VRanges and VCF classes encode different types of information and are semantically incompatible. While methods exist for converting a VCF object to a VRanges and vice versa, information is lost in the transformation. There is no way to collapse multiple rows of a VRanges at the same genomic position and accurately represent missing data. For this reason, it is not reasonable to assume that an object resulting from multiple conversions (VRanges -> VCF -> VRanges) will be equivalent to the original.

### Constructors

  `VRanges(seqnames = Rle(), ranges = IRanges(), ref = character(), alt = NA_character_, totalDepth = NA_integer_, refDepth = NA_integer_, altDepth = NA_integer_, ..., sampleNames = NA_character_, softFilterMatrix = FilterMatrix(matrix(nrow = length(gr), ncol = 0L), FilterRules()), hardFilters = FilterRules())`: Creates a VRanges object.

  `seqnames`

    Rle object, character vector, or factor containing the sequence names.

  `ranges`

    IRanges object containing the ranges.

  `ref`

    character vector, containing the reference allele.

  `alt`

    character vector/Rle, containing the alternative allele (NA allowed).

  `totalDepth`

    integer vector/Rle, containing the total read depth (NA allowed).

  `refDepth`

    integer vector/Rle, containing the reference read depth (NA allowed).

  `altDepth`

    integer vector/Rle, containing the reference read depth (NA allowed).

  `...`

    Arguments passed to the `GRanges` constructor.

  `sampleNames`

    character/factor vector/Rle, containing the sample names (NA allowed).

  `softFilterMatrix`

    a matrix (typically a `FilterMatrix`) of dimension variant by filter, with logical values indicating whether a variant passed the filter.

  `hardFilters`

    a `FilterRules`, containing the filters that have already been applied to subset the object to its current state.

  `makeVRangesFromGRanges(gr, ref.field="ref", alt.field="alt", totalDepth.field="totalDepth", refDepth.field="refDepth", altDepth.field="altDepth", sampleNames.field="sampleNames", keep.extra.columns=TRUE)`: Creates a VRanges object from a GRanges.

  `gr`

    A GenomicRanges object.

  `ref.field`

    The `character(1)` name of the GRanges metadata column to be used as the VRanges `ref` field.

  `alt.field`

    The `character(1)` name of the GRanges metadata column to be used as the VRanges `alt` field.

  `totalDepth.field`

    The `character(1)` name of the GRanges metadata column to be used as the VRanges `totalDepth` field.

  `refDepth.field`

    The `character(1)` name of the GRanges metadata column to be used as the VRanges `refDepth` field.

  `altDepth.field`

    The `character(1)` name of the GRanges metadata column to be used as the VRanges `altDepth` field.

  `sampleNames.field`

    The `character(1)` name of the GRanges metadata column to be used as the VRanges `sampleNames` field.

  `keep.extra.columns`

    `TRUE` (the default) or `FALSE`. If `TRUE`, then the columns in `gr` that are not used to form the VRanges are retained as metadata columns. Otherwise, they will be ignored.

### Coercion

These functions/methods coerce objects to and from `VRanges`:

  `asVCF(x, info = character(), filter = character(), meta = character())`: Creates a VCF object from a VRanges object. The following gives the mapping from VRanges components to VCF:

  seqnames(x)

    CHROM column

  start(x)

    POS column

  names(x)

    ID column

  ref(x)

    REF column

  alt(x)

    ALT column

  totalDepth(x)

    DP in FORMAT column

  altDepth(x), refDepth(x)

    AD in FORMAT column

  sampleNames(x)

    Names the sample columns

  softFilterMatrix(x)

    FT in FORMAT column, except filters named in `filter` argument, which are considered per-position and placed in the FILTER column

  hardFilters(x)

    Not yet exported

  mcols(x)

    Become fields in the FORMAT column; unless they are named in the `info` argument, in which case they are considered per-position and placed in the INFO column

  metadata(x)

    If named in the `meta` argument, output in the VCF header; a component is required to be coercible to a character vector of length one.

  Note that `identical(x, as(as(x, "VCF"), "VRanges"))` generally return `FALSE`. During coercion to VCF, the "geno" components are reshaped into matrix form, with NAs filling the empty cells. The reverse coercion will not drop the NA values, so rows are added to the new VRanges. All logical values will become integers in VCF, and there is no automatic way of regenerating the logical column with the reverse coercion. There are many other cases of irreversibility.

  `as(from, "VCF")`: Like calling `asVCF(from)`.

  `as(from, "VRanges")`: When `from` is a `VCF` this coercion is essentially the inverse of `asVCF`. Information missing in the VCF is imputed as NA.

  When `from` is a `GRanges`, metadata columns of `ref`, `alt`, `refDepth`, `altDepth`, `totalDepth` and `sampleNames` are transfered to the `VRanges` object. Additional metadata columns in the `GRanges` can be retained or dropped with `keep.extra.columns`. See also `makeVRangesFromGRanges`.

### Accessors

In addition to all of the `GRanges` accessors, `VRanges` provides the following, where `x` is a VRanges object.

  `alt(x), alt(x) <- value`: Get or set the alt allele (character).

  `ref(x), ref(x) <- value`: Get or set the ref allele (character).

  `altDepth(x), altDepth(x) <- value`: Get or set the alt allele read depth (integer).

  `refDepth(x), refDepth(x) <- value`: Get or set the ref allele read depth (integer).

  `totalDepth(x), totalDepth(x) <- value`: Get or set the total read depth (integer).

  `altFraction(x)`: Returns `altDepth(x)/totalDepth(x)` (numeric).

  `sampleNames(x), sampleNames(x) <- value`: Get or set the sample names (character/factor).

  `softFilterMatrix(x), softFilterMatrix(x) <- value`: Gets or sets the soft filter matrix (any matrix, but ideally a `FilterMatrix`).

  `resetFilter(x)`: Removes all columns from `softFilterMatrix`.

  `called(x)`: Returns whether all filter results in `softFilterMatrix(x)` are `TRUE` for each variant.

  `hardFilters(x), hardFilters(x) <- value`: Gets or sets the hard filters (those applied to yield the current subset).

### Utilities and Conveniences

  `match(x)`: Like GRanges `match`, except matches on the combination of chromosome, start, width, and alt.

  `tabulate(bin)`: Finds `unique(bin)` and counts how many times each unique element occurs in `bin`. The result is stored in `mcols(bin)$sample.count`.

  `softFilter(x, filters, ...)`: applies the `FilterRules` in `filters` to `x`, storing the results in `softFilterMatrix`.

### Input/Output to/from VCF

  `writeVcf(obj, filename, ...)`: coerces to a VCF object and writes it to a file; see `writeVcf`.

  `readVcfAsVRanges(x, genome, param = ScanVcfParam(), ...)`: Reads a VCF `x` directly into a `VRanges`; see `readVcf` for details on the arguments. `readVcfAsVRanges` is an alternative syntax to

        as(readVcf(), "VRanges")

  NOTE: By default all INFO and FORMAT fields are read in with `ScanVcfParam()`. The minimal information needed to create the `VRanges` can be specified as follows:

        ScanVcfParam(fixed = "ALT", info = NA, geno = "AD"))

### Variant Type

Functions to identify variant type include isSNV, isInsertion, isDeletion, isIndel, isSubstitution and isTransition. See the ?`isSNV` man page for details.

### Author(s)

Michael Lawrence. `makeVRangesFromGRanges` was contributed by Thomas Sandmann.

### See Also

VRangesList, a list of `VRanges`; `bam_tally` in the gmapR package, which generates a `VRanges`.

### Examples

    ## construction
    vr <- VRanges(seqnames = c("chr1", "chr2"),
                  ranges = IRanges(c(1, 10), c(5, 20)),
                  ref = c("T", "A"), alt = c("C", "T"),
                  refDepth = c(5, 10), altDepth = c(7, 6),
                  totalDepth = c(12, 17), sampleNames = letters[1:2],
                  hardFilters =
                    FilterRules(list(coverage = function(x) totalDepth > 10)),
                  softFilterMatrix =
                    FilterMatrix(matrix = cbind(depth = c(TRUE, FALSE)),
                                 FilterRules(depth = function(x) altDepth(x) > 6)),
                  tumorSpecific = c(FALSE, TRUE))

    ## simple accessors
    ref(vr)
    alt(vr)
    altDepth(vr)
    vr$tumorSpecific
    called(vr)

    ## coerce to VCF and write
    vcf <- as(vr, "VCF")
    ## writeVcf(vcf, "example.vcf")
    ## or just
    ## writeVcf(vr, "example.vcf")

    ## other utilities
    match(vr, vr[2:1])

[Package VariantAnnotation version 1.38.0 Index]