signatureGeneHeatmap {TBSignatureProfiler} | R Documentation

## Plot a heatmap of a single signature score with individual gene expression levels.

### Description

This function takes the profiled gene expression data for a single signature and creates a heatmap based on the expression scores.

### Usage

    signatureGeneHeatmap(
      inputData,
      useAssay,
      sigGenes,
      name = "Signature",
      signatureColNames = NULL,
      annotationColNames = NULL,
      scale = TRUE,
      showColumnNames = TRUE,
      showRowNames = TRUE,
      colList = list(),
      colorSets = c("Set1", "Set2", "Set3", "Pastel1", "Pastel2", "Accent", "Dark2",
        "Paired"),
      choose_color = c("blue", "gray95", "red"),
      column_order = NULL,
      ...
    )

### Arguments

`inputData` | a `SummarizedExperiment` object containing the profiled signature data and annotation data as columns in the `colData`. Required.
`useAssay` | a character string specifying the assay to use for the gene expression data. Required.
`sigGenes` | a vector identifying the genes in the signature to use in the heatmap. For inbuilt signatures, you can use `TBsignatures` (e.g., `TBsignatures[["ACS_COR"]]`). Required.
`name` | a character string with the plot title of the heatmap. The default is `"Signatures"`.
`signatureColNames` | a vector of the column names in the `colData` that contain the signature data. Required.
`annotationColNames` | a vector of the column names in the `colData` that contain the annotation data. If `NULL`, no annotation bar besides those of the scoring algorithms will be drawn on the heatmap. The default is `NULL`.
`scale` | logical. Setting `scale = TRUE` scales the signature data. The default is `TRUE`.
`showColumnNames` | logical. Setting `showColumnNames = TRUE` will show the column names (i.e. sample names) on the heatmap. The default is `TRUE`.
`showRowNames` | logical. Setting `showColumnNames = TRUE` will show the row names (i.e. signature names) on the heatmap. The default is `TRUE`.
`colList` | a named `list` of named vectors specifying custom color information to pass to `ComplexHeatmap::Heatmap()`. The list should have as many elements as there are annotation columns and gene signatures (i.e. `sigGenes`), and each element name should correspond exactly with the name of each annotation column/signature. The colors in the vector elements should be named according to the levels of the factor in that column's annotation data if the annotation is discrete, or it should be produced with `circlize::colorRamp2` if the annotation/gene is continuous. By default, `ColorBrewer` color sets will be used. See the the parameter `colorSets` for additional details.
`colorSets` | a vector of names listing the color sets in the order that they should be used in creating the heatmap. By default, this function will use the color sets in the order listed in `Usage` for annotation information. You may replace the default with the same collection of sets in order that you want to use them, or provide custom color sets with the `colList` parameter.
`choose_color` | a vector of color names to be interpolated for the heatmap gradient, or a `colorRamp` function produced by `circlize::colorRamp2`. The default is `c("blue", "gray95", "red")`.
`column_order` | a vector of character strings indicating the order in which to manually arrange the heatmap columns. Default is `NULL`, such that column order is automatically determined via clustering.
`...` | Additional arguments to be passed to `ComplexHeatmap::Heatmap()`.

### Value

A `ComplexHeatmap` plot.

### Examples

    library(SummarizedExperiment)
    # Generate some artificial data that shows a difference in Zak_RISK_16
    mat_testdata <- rbind(matrix(c(rnorm(80), rnorm(80) + 5), 16, 10,
                                 dimnames = list(TBsignatures$Zak_RISK_16,
                                                 paste0("sample", seq_len(10)))),
                          matrix(rnorm(1000), 100, 10,
                                 dimnames = list(paste0("gene", seq_len(100)),
                                                 paste0("sample", seq_len(10)))))

    # Create a SummarizedExperiment object that contains the data
    testdataSE <- SummarizedExperiment(assays = SimpleList(data = mat_testdata),
                                       colData = DataFrame(sample =
                                                           c(rep("down", 5),
                                                             rep("up", 5))))
    # Run profiler using GSVA and ssGSEA on Zak_RISK_16
    res <- runTBsigProfiler(testdataSE, useAssay = "data",
                            signatures = TBsignatures["Zak_RISK_16"],
                            algorithm = c("GSVA", "ssGSEA"), parallel.sz = 1,
                            combineSigAndAlgorithm = TRUE)

    # Plot a heatmap of signature genes and pathway predictions
    signatureGeneHeatmap(res, useAssay = "data",
                         sigGenes = TBsignatures[["Zak_RISK_16"]],
                         signatureColNames = c("GSVA_Zak_RISK_16",
                                               "ssGSEA_Zak_RISK_16"),
                         annotationColNames = c("sample"), showColumnNames = FALSE,
                         name = "Zak_RISK_16")

[Package TBSignatureProfiler version 1.4.11 Index]