joinAssays {QFeatures} | R Documentation

## Join assays in a QFeatures object

### Description

This function applies a full-join type of operation on 2 or more assays in a `QFeatures` instance.

### Usage

    joinAssays(x, i, name = "joinedAssay")

### Arguments

`x` | An instance of class QFeatures.
`i` | The indices or names of al least two assays to be joined.
`name` | A `character(1)` naming the new assay. Default is `joinedAssay`. Note that the function will fail if there's already an assay with `name`.

### Details

The rows to be joined are chosen based on the rownames of the respective assays. It is the user's responsability to make sure these are meaningful, such as for example refering to unique peptide sequences or proteins.

The join operation acts along the rows and expects the samples (columns) of the assays to be disjoint, i.e. the assays mustn't share any samples. Rows that aren't present in an assay are set to `NA` when merged.

The `rowData` slots are also joined. However, only columns that are shared and that have the same values for matching columns/rows are retained. For example of a feature variable `A` in sample `S1` contains value `a1` and variable `A` in sample `S2` in a different assay contains `a2`, then the feature variable `A` is dropped in the merged assay.

The joined assay is linked to its parent assays through an `AssayLink` object. The link between the child assay and the parent assays is based on the assay row names, just like the procedure for joining the parent assays.

### Value

A `QFeatures` object with an additional assay.

### Author(s)

Laurent Gatto

### Examples

    ## -----------------------------------------------
    ## An example QFeatures with 3 assays to be joined
    ## -----------------------------------------------
    data(feat2)
    feat2

    feat2 <- joinAssays(feat2, 1:3)

    ## Individual assays to be joined, each with 4 samples and a
    ## variable number of rows.
    assay(feat2[[1]])
    assay(feat2[[2]])
    assay(feat2[[3]])

    ## The joined assay contains 14 rows (corresponding to the union
    ## of those in the initial assays) and 12 samples
    assay(feat2[["joinedAssay"]])

    ## The individual rowData to be joined.
    rowData(feat2[[1]])
    rowData(feat2[[2]])
    rowData(feat2[[3]])

    ## Only the 'Prot' variable is retained because it is shared among
    ## all assays and the values and coherent across samples (the
    ## value of 'Prot' for row 'j' is always 'Pj'). The variable 'y' is
    ## missing in 'assay1' and while variable 'x' is present is all
    ## assays, the values for the shared rows are different.
    rowData(feat2[["joinedAssay"]])

[Package QFeatures version 1.2.0 Index]