exp3_OHT_ESR1 {qPLEXanalyzer} | R Documentation

## exp3_OHT_ESR1 dataset

### Description

Three ER qPLEX-RIME (10plex) experiments were performed to investigate the dynamics of the ER complex assembly upon 4-hydrotamoxifen (OHT) treatment at 2h, 6h and 24h or at 24h post-treatment with the drug-vehicle alone (ethanol). Two biological replicates of each condition were included in each experiment to finally consider a total of six replicates per time point. Additionally, MCF7 cells were treated with OHT or ethanol and cross-linked at 24h post-treatment in each experiment to be used for mock IgG pull-downs and to enable discrimination of non-specific binding in the same experiment. This is a timecourse experiment to study the effect of tamoxifen in ER interactome using qPLEX-RIME method.

### Format

An object of class `list` related to peptides quantification. It consists of qPLEX-RIME data from three experimental runs. Each run contains 10 samples divided into five conditions (IgG, vehicle, tam.2h, tam.6h and tam.24h).

### Value

An object of class `list` related to peptides quantification.

[Package qPLEXanalyzer version 1.8.2 Index]