GenotypeMatrix-class {podkat} | R Documentation

## Class `GenotypeMatrix`

### Description

S4 class for storing genotypes efficiently as column-oriented sparse matrices along with variant info

### Details

This class stores genotypes as a column-oriented sparse numeric matrix, where rows correspond to samples and columns correspond to variants. This is accomplished by extending the `dgCMatrix` class from which this class inherits all slots. Information about variants is stored in an additional slot named `variantInfo`. This slot must be of class `VariantInfo` and have exactly as many elements as the genotype matrix has columns. The `variantInfo` slot has a dedicated metadata column named “MAF” that contains the minor allele frequencies (MAFs) of the variants. For convenience, accessor functions `variantInfo` and `MAF` are available (see below).

Objects of this class should only be created and manipulated by the constructors and accessors described below, as only these methods ensure the integrity of the created objects. Direct modification of object slots is strongly discouraged!

### Constructors

See help pages `genotypeMatrix` and `readGenotypeMatrix`.

### Methods

show

  `signature(object="GenotypeMatrix")`: displays the matrix dimensions (i.e. the number of samples and variants) along with some basic statistics of the minor allele frequency (MAF).

### Accessors

variantInfo

  `signature(object="GenotypeMatrix")`: returns variant information as a `VariantInfo` object.

MAF

  `signature(object="GenotypeMatrix")`: returns a numeric vector with the minor allele frequencies (MAFs).

Row and column names can be set and get as usual for matrix-like objects with `rownames` and `colnames`, respectively. When setting the column names of a `GenotypeMatrix` object, both the names of the variant info (slot `variantInfo`) and the column names of the matrix are set.

### Subsetting

In the following code snippets, `x` is a `GenotypeMatrix` object.

  `x[i, ]`: returns a `GenotypeMatrix` object that only contains the samples selected by the index vector `i`

  `x[, j]`: returns a `GenotypeMatrix` object that only contains the variants selected by the index vector `j`

  `x[i, j]`: returns a `GenotypeMatrix` object that only contains the samples selected by the index vector `i` and the variants selected by the index vector `j`

None of these subsetting functions support a `drop` argument. As soon as a `drop` argument is supplied, no matter whether `TRUE` or `FALSE`, all variant information is stripped off and a `dgCMatrix` object is returned.

By default, MAFs are not altered by subsetting samples. However, if the optional argument `recomputeMAF` is set to `TRUE` (the default is `FALSE`), MAFs are recomputed for the resulting subsetted genotype matrix as described in `genotypeMatrix`. The ploidy for computing MAFs can be controlled by the optional `ploidy` argument (the default is 2).

### Author(s)

Ulrich Bodenhofer bodenhofer@bioinf.jku.at

### References

http://www.bioinf.jku.at/software/podkat

### See Also

`dgCMatrix`, `VariantInfo`, `genotypeMatrix`, `readGenotypeMatrix`

### Examples

    ## create a toy example
    A <- matrix(rbinom(50, 2, prob=0.2), 5, 10)
    sA <- as(A, "dgCMatrix")
    pos <- sort(sample(1:10000, ncol(A)))
    seqname <- "chr1"

    ## variant with 'GRanges' object
    gr <- GRanges(seqnames=seqname, ranges=IRanges(start=pos, width=1))
    gtm <- genotypeMatrix(A, gr)
    gtm
    as.matrix(gtm)
    variantInfo(gtm)
    MAF(gtm)

    ## variant with 'pos' and 'seqnames' object
    genotypeMatrix(sA, pos, seqname)

    ## variant with 'seqname:pos' strings passed through 'pos' argument
    spos <- paste(seqname, pos, sep=":")
    spos
    genotypeMatrix(sA, spos)

    ## read data from VCF file using 'readVcf()' from the 'VariantAnnotation'
    ## package
    if (require(VariantAnnotation))
    {
        vcfFile <- system.file("examples/example1.vcf.gz", package="podkat")
        sp <- ScanVcfParam(info=NA, genome="GT", fixed=c("ALT", "FILTER"))
        vcf <- readVcf(vcfFile, genome="hgA", param=sp)
        rowRanges(vcf)

        ## call constructor for 'VCF' object
        gtm <- genotypeMatrix(vcf)
        gtm
        variantInfo(gtm)

        ## alternatively, extract information from 'VCF' object and use
        ## variant with character matrix and 'GRanges' positions
        ## note that, in 'VCF' objects, rows correspond to variants and
        ## columns correspond to samples, therefore, we have to transpose the
        ## genotype
        gt <- t(geno(vcf)$GT)
        gt[1:5, 1:5]
        gr <- rowRanges(vcf)
        gtm <- genotypeMatrix(gt, gr)
        as.matrix(gtm[1:20, 1:5, recomputeMAF=TRUE])
    }

[Package podkat version 1.22.0 Index]