PlotGroups {maSigPro} | R Documentation

## Function for plotting gene expression profile at different experimental groups

### Description

This function displays the gene expression profile for each experimental group in a time series gene expression experiment.

### Usage

    PlotGroups(data, edesign = NULL, time = edesign[,1], groups = edesign[,c(3:ncol(edesign))],
               repvect = edesign[,2], show.fit = FALSE, dis = NULL, step.method = "backward",
               min.obs = 2, alfa = 0.05, nvar.correction = FALSE, summary.mode = "median", show.lines = TRUE, groups.vector = NULL,
               xlab = "Time", ylab = "Expression value", cex.xaxis = 1, ylim = NULL, main = NULL, cexlab = 0.8, legend = TRUE, sub = NULL, item = NULL)

### Arguments

`data` | vector or matrix containing the gene expression data
`edesign` | matrix describing experimental design. Rows must be arrays and columns experiment descriptors
`time` | vector indicating time assigment for each array
`groups` | matrix indicating experimental group to which each array is assigned
`repvect` | index vector indicating experimental replicates
`show.fit` | logical indicating whether regression fit curves must be plotted
`dis` | regression design matrix
`step.method` | stepwise regression method to fit models for cluster mean profiles. It can be either `"backward"`, `"forward"`, `"two.ways.backward"` or `"two.ways.forward"`
`min.obs` | minimal number of observations for a gene to be included in the analysis
`alfa` | significance level used for variable selection in the stepwise regression
`nvar.correction` | argument for correcting stepwise regression significance level. See `T.fit`
`summary.mode` | the method to condensate expression information when more than one gene is present in the data. Possible values are `"representative"` and `"median"`
`show.lines` | logical indicating whether a line must be drawn joining plotted data points for reach group
`groups.vector` | vector indicating experimental group to which each variable belongs
`xlab` | label for the x axis
`ylab` | label for the y axis
`cex.xaxis` | graphical parameter maginfication to be used for x axis in plotting functions
`ylim` | range of the y axis
`main` | plot main title
`cexlab` | graphical parameter maginfication to be used for x axis label in plotting functions
`legend` | logical indicating whether legend must be added when plotting profiles
`sub` | plot subtitle
`item` | Name of the analysed items to show

### Details

To compute experimental groups either a edesign object must be provided, or separate values must be given for the `time`, `repvect` and `groups` arguments.

When data is a matrix, the average expression value is displayed.

When there are array replicates in the data (as indicated by `repvect`), values are averaged by `repvect`.

PlotGroups plots one single expression profile for each experimental group even if there are more that one genes in the data set. The way data is condensated for this is given by `summary.mode`. When this argument takes the value `"representative"`, the gene with the lowest distance to all genes in the cluster will be plotted. When the argument is `"median"`, then median expression value is computed.

When `show.fit` is `TRUE` the stepwise regression fit for the data will be computed and the regression curves will be displayed.

If data is a matrix of genes and `summary.mode` is `"median"`, the regression fit will be computed for the median expression value.

### Value

Plot of gene expression profiles by-group.

### Author(s)

Ana Conesa, aconesa@cipf.es; Maria Jose Nueda, mj.nueda@ua.es

### References

Conesa, A., Nueda M.J., Alberto Ferrer, A., Talon, T. 2005. maSigPro: a Method to Identify Significant Differential Expression Profiles in Time-Course Microarray Experiments.

### See Also

`PlotProfiles`

### Examples

    #### GENERATE TIME COURSE DATA
    ## generate n random gene expression profiles of a data set with
    ## one control plus 3 treatments, 3 time points and r replicates per time point.

    tc.GENE <- function(n, r,
                 var11 = 0.01, var12 = 0.01,var13 = 0.01,
                 var21 = 0.01, var22 = 0.01, var23 =0.01,
                 var31 = 0.01, var32 = 0.01, var33 = 0.01,
                 var41 = 0.01, var42 = 0.01, var43 = 0.01,
                 a1 = 0, a2 = 0, a3 = 0, a4 = 0,
                 b1 = 0, b2 = 0, b3 = 0, b4 = 0,
                 c1 = 0, c2 = 0, c3 = 0, c4 = 0)
    {

      tc.dat <- NULL
      for (i in 1:n) {
        Ctl <- c(rnorm(r, a1, var11), rnorm(r, b1, var12), rnorm(r, c1, var13))  # Ctl group
        Tr1 <- c(rnorm(r, a2, var21), rnorm(r, b2, var22), rnorm(r, c2, var23))  # Tr1 group
        Tr2 <- c(rnorm(r, a3, var31), rnorm(r, b3, var32), rnorm(r, c3, var33))  # Tr2 group
        Tr3 <- c(rnorm(r, a4, var41), rnorm(r, b4, var42), rnorm(r, c4, var43))  # Tr3 group
        gene <- c(Ctl, Tr1, Tr2, Tr3)
        tc.dat <- rbind(tc.dat, gene)
      }
      tc.dat
    }

    ## create 10 genes with profile differences between Ctl, Tr2, and Tr3 groups
    tc.DATA <- tc.GENE(n = 10,r = 3, b3 = 0.8, c3 = -1, a4 = -0.1, b4 = -0.8, c4 = -1.2)
    rownames(tc.DATA) <- paste("gene", c(1:10), sep = "")
    colnames(tc.DATA) <- paste("Array", c(1:36), sep = "")

    #### CREATE EXPERIMENTAL DESIGN
    Time <- rep(c(rep(c(1:3), each = 3)), 4)
    Replicates <- rep(c(1:12), each = 3)
    Ctl <- c(rep(1, 9), rep(0, 27))
    Tr1 <- c(rep(0, 9), rep(1, 9), rep(0, 18))
    Tr2 <- c(rep(0, 18), rep(1, 9), rep(0, 9))
    Tr3 <- c(rep(0, 27), rep(1, 9))

    PlotGroups (tc.DATA, time = Time, repvect = Replicates, groups = cbind(Ctl, Tr1, Tr2, Tr3))

[Package maSigPro version 1.62.0 Index]