convertGenomesFromVRanges {decompTumor2Sig} | R Documentation

## Convert genomes from a `VRanges` object

### Description

'convertGenomesFromVRanges()' converts the SNVs of a single tumor genome (sample) or a set of genomes from a `VRanges` object (package `VariantAnnotation`) and determines the mutation frequencies according to a specific model of mutational signatures (Alexandrov or Shiraishi), such that the resulting format can be used as genomes input for `decomposeTumorGenomes`.

### Usage

    convertGenomesFromVRanges(vranges, numBases=5, type="Shiraishi",
    trDir=TRUE, enforceUniqueTrDir=TRUE,
    refGenome=BSgenome.Hsapiens.UCSC.hg19::BSgenome.Hsapiens.UCSC.hg19,
    transcriptAnno=
    TxDb.Hsapiens.UCSC.hg19.knownGene::TxDb.Hsapiens.UCSC.hg19.knownGene,
    verbose=TRUE)

### Arguments

`vranges` | (Mandatory) The `VRanges` object which specifies the mutations.
`numBases` | (Mandatory) Total number of bases (mutated base and flanking bases) to be used for sequence patterns. Must be odd. Default: 5
`type` | (Mandatory) Signature model or type (`"Alexandrov"` or `"Shiraishi"`). Default: `"Shiraishi"`
`trDir` | (Mandatory) Specifies whether the transcription direction is taken into account in the signature model. If so, only mutations within genomic regions with a defined transcription direction can be considered. Default: `TRUE`
`enforceUniqueTrDir` | (Optional) Used only if `trDir` is `TRUE`. If `enforceUniqueTrDir` is TRUE (default), then mutations which map to a region with multiple overlapping genes with opposing transcription directions will be excluded from the analysis. If `FALSE`, the transcript direction encountered first in the transcript database (see `transcriptAnno`) is assigned to the mutation. The latter was the behavior until version 1.3.5 of `decompTumor2Sig` and is also the behavior of `pmsignature`. However, it is preferable to exclude these mutations from the count (default) because from mutation data alone it cannot be inferred which of the two genes has the higher transcriptional activity which might potentially be linked to the occurrence of the mutation. (If you are unsure, use the default setting; this option exists mostly for backward compatibility with older versions.)
`refGenome` | (Mandatory) The reference genome (`BSgenome`) needed to extract sequence patterns. Default: `BSgenome` object for hg19.
`transcriptAnno` | (Optional) Transcript annotation (`TxDb` object) used to determine the transcription direction. This is required only if `trDir` is `TRUE`. Default: `TxDb` object for hg19.
`verbose` | (Optional) Print information about reading and processing the mutation data. Default: `TRUE`

### Value

A list containing the genomes in terms of frequencies of the mutated sequence patterns. This list of genomes can be used for `decomposeTumorGenomes`.

### Author(s)

Rosario M. Piro
Politecnico di Milano
Maintainer: Rosario M. Piro
E-Mail: <rmpiro@gmail.com> or <rosariomichael.piro@polimi.it>

### References

http://rmpiro.net/decompTumor2Sig/
Krueger, Piro (2019) decompTumor2Sig: Identification of mutational signatures active in individual tumors. BMC Bioinformatics 20(Suppl 4):152.

### See Also

`decompTumor2Sig`
`decomposeTumorGenomes`
`readGenomesFromVCF`
`readGenomesFromMPF`
`getGenomesFromMutFeatData`

### Examples

    ### load the reference genome and the transcript annotation database
    refGenome <- BSgenome.Hsapiens.UCSC.hg19::BSgenome.Hsapiens.UCSC.hg19
    transcriptAnno <-
      TxDb.Hsapiens.UCSC.hg19.knownGene::TxDb.Hsapiens.UCSC.hg19.knownGene

    ### take the breast cancer genomes from Nik-Zainal et al (PMID: 22608084)
    gfile <- system.file("extdata",
             "Nik-Zainal_PMID_22608084-VCF-convertedfromMPF.vcf.gz",
             package="decompTumor2Sig")

    ### get the corresponding VRanges object (using the VariantAnnotation
    ### package)
    library(VariantAnnotation)
    vr <- readVcfAsVRanges(gfile, genome="hg19")

    ### convert the VRanges object to the decompTumor2Sig format
    genomes <- convertGenomesFromVRanges(vr, numBases=5, type="Shiraishi",
             trDir=TRUE, enforceUniqueTrDir=TRUE, refGenome=refGenome,
             transcriptAnno=transcriptAnno, verbose=FALSE)

[Package decompTumor2Sig version 2.6.0 Index]