getDistrs {casper} | R Documentation

## Compute fragment start and fragment length distributions

### Description

Compute fragment start distributions by using reads aligned to genes with only one annotated variant. Estimate fragment length distribution using fragments aligned to long exons (>1000nt). Fragment length is defined as the distance between the start of the left-end read and the end of the right-end read.

### Usage

    getDistrs(DB, bam, pbam, islandid=NULL, verbose=FALSE, nreads=4*10^6,
    readLength, min.gt.freq = NULL, tgroups=5, mc.cores=1)

### Arguments

`DB` | Annotated genome. Object of class `knownGenome` as returned by `procGenome`.
`bam` | Aligned reads, as returned by `scanBam`. It must be a list with elements 'qname', 'rname', 'pos' and 'mpos'. Ignored when argument `pbam` is specified.
`pbam` | Processed BAM object of class `procBam`, as returned by function `procBam`. Arguments `bam` and `readLength` are ignored when `pbam` is specified.
`islandid` | Island IDs of islands to be used in the read start distribution calculations (defaults to genes with only one annotated variant)
`verbose` | Set to `TRUE` to print progress information.
`nreads` | To speed up computations, only the first `nreads` are used to obtain the estimates. The default value of 4 milions usually gives highly precise estimates.
`readLength` | Read length in bp, e.g. in a paired-end experiment where 75bp are sequenced on each end one would set `readLength=75`.
`min.gt.freq` | The target distributions cannot be estimated with precision for gene types that are very unfrequent. Gene types with relative frequency below `min.gt.freq` are merged, e.g. `min.gt.freq=0.05` means gene types making up for 5% of the genes in DB will be combined and a single read start and length distribution will be estimated for all of them.
`tgroups` | As an alternative to `min.gt.freq` you may specify the maximum number of distinct gene types to consider. A separate estimate will be obtained for the `tgroups` with highest frequency, all others will be combined.
`mc.cores` | Number of cores to use for parallel processing

### Value

An object of class readDistrs with slots:

`lenDis` | Table with number of fragments with a given length
`stDis` | Cumulative distribution function (object of type closure) for relative start position

### Author(s)

Camille Stephan-Otto Attolini, David Rossell

### Examples

    data(K562.r1l1)
    data(hg19DB)
    bam0 <- rmShortInserts(K562.r1l1, isizeMin=100)

    distrs <- getDistrs(hg19DB,bam=bam0,readLength=75)

    #Fragment length distribution
    plot(distrs,'fragLength')

    #Fragment start distribution (relative to transcript length)
    plot(distrs,'readSt')

[Package casper version 2.24.2 Index]