findMapORFs {ORFik} | R Documentation

## Find ORFs and immediately map them to their genomic positions.

### Description

This function can map spliced ORFs. It finds ORFs on the sequences of interest, but returns relative positions to the positions of 'grl' argument. For example, 'grl' can be exons of known transcripts (with genomic coordinates), and 'seq' sequences of those transcripts, in that case, this function will return genomic coordinates of ORFs found on transcript sequences.

### Usage

    findMapORFs(
      grl,
      seqs,
      startCodon = startDefinition(1),
      stopCodon = stopDefinition(1),
      longestORF = TRUE,
      minimumLength = 0,
      groupByTx = FALSE
    )

### Arguments

`grl` | (`GRangesList`) of sequences to search for ORFs, probably in genomic coordinates
`seqs` | (DNAStringSet or character vector) - DNA/RNA sequences to search for Open Reading Frames. Can be both uppercase or lowercase. Easiest call to get seqs if you want only regions from a fasta/fasta index pair is: seqs = ORFik:::txSeqsFromFa(grl, faFile), where grl is a GRanges/List of search regions and faFile is a `FaFile`.
`startCodon` | (character vector) Possible START codons to search for. Check `startDefinition` for helper function.
`stopCodon` | (character vector) Possible STOP codons to search for. Check `stopDefinition` for helper function.
`longestORF` | (logical) Default TRUE. Keep only the longest ORF per unique stopcodon: (seqname, strand, stopcodon) combination, Note: Not longest per transcript! You can also use function `longestORFs` after creation of ORFs for same result.
`minimumLength` | (integer) Default is 0. Which is START + STOP = 6 bp. Minimum length of ORF, without counting 3bps for START and STOP codons. For example minimumLength = 8 will result in size of ORFs to be at least START + 8*3 (bp) + STOP = 30 bases. Use this param to restrict search.
`groupByTx` | logical (default: FALSE), should output GRangesList be grouped by exons per ORF (TRUE) or by orfs per transcript (FALSE)?

### Details

This function assumes that 'seq' is in widths relative to 'grl', and that their orders match. 1st seq is 1st grl object, etc.

See vignette for real life example.

### Value

A GRangesList of ORFs.

### See Also

Other findORFs: `findORFsFasta()`, `findORFs()`, `findUORFs()`, `startDefinition()`, `stopDefinition()`

### Examples

    # First show simple example using findORFs
    # This sequence has ORFs at 1-9 and 4-9
    seqs <- DNAStringSet("ATGATGTAA") # the dna transcript sequence
    findORFs(seqs)
    # lets assume that this sequence comes from two exons as follows
    # Then we need to use findMapORFs instead of findORFs,
    #  for splicing information
    gr <- GRanges(seqnames = rep("1", 2), # chromosome 1
                  ranges = IRanges(start = c(21, 10), end = c(23, 15)),
                  strand = rep("-", 2), names = rep("tx1", 2))
    grl <- GRangesList(tx1 = gr)
    findMapORFs(grl, seqs) # ORFs are properly mapped to its genomic coordinates

    grl <- c(grl, grl)
    names(grl) <- c("tx1", "tx2")
    findMapORFs(grl, c(seqs, seqs))
    # More advanced example and how to save sequences found in vignette

[Package ORFik version 1.10.13 Index]