get_df_long {DEP} | R Documentation

## Generate a long data.frame from a SummarizedExperiment

### Description

`get_df_long` generate a wide data.frame from a SummarizedExperiment.

### Usage

    get_df_long(se)

### Arguments

`se` | SummarizedExperiment, Proteomics data (output from `make_se()` or `make_se_parse()`).

### Value

A data.frame object containing all data in a wide format, where each row represents a single measurement.

### Examples

    # Load example
    data <- UbiLength
    data <- data[data$Reverse != "+" & data$Potential.contaminant != "+",]
    data_unique <- make_unique(data, "Gene.names", "Protein.IDs", delim = ";")

    # Make SummarizedExperiment
    columns <- grep("LFQ.", colnames(data_unique))
    exp_design <- UbiLength_ExpDesign
    se <- make_se(data_unique, columns, exp_design)

    # Filter, normalize and impute missing values
    filt <- filter_missval(se, thr = 0)
    norm <- normalize_vsn(filt)
    imputed <- impute(norm, fun = "MinProb", q = 0.01)

    # Test for differentially expressed proteins
    diff <- test_diff(imputed, "control", "Ctrl")
    dep <- add_rejections(diff, alpha = 0.05, lfc = 1)

    # Get a long data.frame
    long <- get_df_long(dep)
    colnames(long)

[Package DEP version 1.12.0 Index]