computeOptimal {ChIPanalyser} | R Documentation

## compute Optimal Parameters

### Description

`ChIPanalyser` contains a set of functions some of which require two parameters known as `lambdaPWM` and as `boundMolecules`. These two paramters are not always known. `computeOptimal` will compute these values by maximising the correlation and minimising the Mean Squared Error between a predicted ChIP-seq-like profile and a real ChIP-seq profile for a given loci.

### Usage

    computeOptimal(genomicProfiles,DNASequenceSet, ChIPScore,chromatinState = NULL,
        parameterOptions = NULL, optimalMethod = "all",rank=FALSE,returnAll=TRUE,
        peakMethod="moving_kernel",cores=1)

### Arguments

`genomicProfiles` | `genomicProfiles` is a `genomicProfiles` object containing at least a Postion Frequency Matrix or a Position Weight Matrix. It is strongly advised to customize this object to increase goodness of fit of the model when compared to real ChIP-seq data.
`DNASequenceSet` | `DNASequenceSet` is a `DNAStringSet` or a `BSgenome` of the full sequence of the organism of interest.
`ChIPScore` | `ChIPScore` is a named list containing ChIP-seq enrichements for each Loci of interest. This Profile should be normalised to a base pair level. In other words, there should be an enrichement score for each base pair of a given Locus.
`chromatinState` | `chromatinState` is a `GRanges` object containing either accesible sites or DNA affinity scores.
`parameterOptions` | `parameterOptions` is a `parameterOptions` object. If this object is not provided (parameterOptions = NULL), a new object will be created internally. However, it is strongly advised to tailor this object to maximise the goodness of fit of the model when compared to ChIP-seq data.
`optimalMethod` | `optimalMethod` is a character string which determines which method for optimal parameter selection should be selected. optimalMethod can be one of the following: pearson, spearman, kendall, ks, fscore, geometric,MSE, or all. Default is set to all.
`rank` | `rank` is a logical value indicating if optimal parameters should be based on rank (parameter combination occuring the most over all regions) or avaerage score (best perfomring combination of paramters on average over all regions selected). DEFAULT = FALSE
`returnAll` | `returnAll` is a logical value indicating if all internal objects should be returned. DEFAULT = TRUE. Internal objects are the following: Occupancy Scores, ChIP like profiles, goodness of fit metrics and optimal paramters. If set to FALSE, `computeOptimal` will only return the optimal parameters.
`peakMethod` | `peakMethod` is a character string of one of the following: c("moving_kernel","truncated_kernel","exact"). If set to moving_kernel, the peaks will be approximated using Rcpp (Default). If set to truncated_kernel, the peaks will be approximated however this method does not require Rcpp. If set to exact, the peaks will not be approximated.
`cores` | `cores` is the number cores that will be used to compute optimal set of parameters.

### Details

In order to backward infer the values of `lambdaPWM` and `boundMolecules`, it is possible to use the `computeOptimal` to find these parameters. It should be noted that this functions requires a ChIP-seq data input. `ChIPScore` (ChIP-seq data). This should be the output of the `processingChIP` function.

### Value

`computeOptimal` returns a list respectivly described as the optimal set of Parameters (lambda - `lambdaPWM` and `boundMolecules`), the optimal matrix (a matrix containing accuracy estimates dependant on the parameter chosen), and finally the chosen parameter. If the parameter that was chosen was "all", then each element of this list will contain the optimal set of parameters, optimal matricies for all of the aforementioned paramters (see `optimalMethod`).

### Author(s)

Patrick C. N. Martin <pm16057@essex.ac.uk>

### References

Zabet NR, Adryan B (2015) Estimating binding properties of transcription factors from genome-wide binding profiles. Nucleic Acids Res., 43, 84–94.

### Examples

    #Data extraction
    data(ChIPanalyserData)
    # path to Position Frequency Matrix
    PFM <- file.path(system.file("extdata",package="ChIPanalyser"),"BCDSlx.pfm")
    #As an example of genome, this example will run on the Drosophila genome

    if(!require("BSgenome.Dmelanogaster.UCSC.dm3", character.only = TRUE)){
        if (!requireNamespace("BiocManager", quietly=TRUE))
            install.packages("BiocManager")
        BiocManager::install("BSgenome.Dmelanogaster.UCSC.dm3")
        }
    library(BSgenome.Dmelanogaster.UCSC.dm3)
    DNASequenceSet <- getSeq(BSgenome.Dmelanogaster.UCSC.dm3)
    eveLocusChip<-processingChIP(eveLocusChip,eveLocus)
    #Building data objects
    GPP <- genomicProfiles(PFM=PFM,BPFrequency=DNASequenceSet)
    OPP <- parameterOptions()
    #Computing Optimal set of Parameters
    optimalParam <- computeOptimal(genomicProfiles = GPP,
        DNASequenceSet = DNASequenceSet,
        ChIPScore = eveLocusChip,
        chromatinState = Access,
        parameterOptions = OPP,
        parameter = "all",
        peakMethod="moving_kernel")

[Package ChIPanalyser version 1.12.0 Index]