createPoolScreenExp {gscreend} | R Documentation

## Create PoolScreenExp Experiment

### Description

Create PoolScreenExp Experiment

### Usage

    createPoolScreenExp(data)

### Arguments

`data` | Input data object containing gRNA level data (SummarizedExperiment)

### Value

object PoolScreenExp object

### Examples

    raw_counts <- read.table(
                            system.file('extdata', 'simulated_counts.txt',
                            package = 'gscreend'),
                            header=TRUE)

    counts_matrix <- cbind(raw_counts$library0, raw_counts$R0_0, raw_counts$R1_0)

    rowData <- data.frame(sgRNA_id = raw_counts$sgrna_id,
    gene = raw_counts$Gene)

    colData <- data.frame(samplename = c('library', 'R1', 'R2'),
    timepoint = c('T0', 'T1', 'T1'))

    library(SummarizedExperiment)
    se <- SummarizedExperiment(assays=list(counts=counts_matrix),
    rowData=rowData, colData=colData)

    # create a PoolScreenExp experiment
    pse <- createPoolScreenExp(se)

[Package gscreend version 1.1.0 Index]