normalizeFrames {flowcatchR} | R Documentation

## Normalize the values of a `Frames` object

### Description

Applies a transformation to the `Frames` object in a way that the intensities throughout the acquisition are normalized overall in term of pixel values sums. It can be used to compensate for example a global change in the illumination values, e.g. due to changed acquisition conditions in experiments that span long timescales.

### Usage

    normalizeFrames(frames, normFun = "median")

### Arguments

`frames` | A `Frames` object to normalize
`normFun` | The normalization function chosen. Can be one of `mean` or `median`

### Value

A `Frames` object with normalized pixel values.

### Author(s)

Federico Marini, marinif@uni-mainz.de, 2014

### Examples

    data(MesenteriumSubset)
    normalizeFrames(MesenteriumSubset,normFun="median")

[Package flowcatchR version 1.21.0 Index]