domainDirections {diffHic} | R Documentation

## Calculate domain directionality

### Description

Collect directionality statistics for domain identification with genomic bins.

### Usage

    domainDirections(files, param, width=50000, span=10)

### Arguments

`files` | a character vector containing paths to the index files generated from each Hi-C library
`param` | a `pairParam` object containing read extraction parameters
`width` | an integer scalar specifying the width of each bin in base pairs
`span` | an integer scalar specifying the distance to consider for up/downstream interactions

### Details

The genome is partitioned into bins of size `width`. For each bin, this function computes the total number of read pairs between that bin and the `span` upstream bins (i.e., those with higher genomic coordinates). This is repeated for the `span` downstream bins, thus yielding two counts (up and down) per bin.

A RangedSummarizedExperiment is returned containing the coordinates of each bin and two matrices of counts, named `"up"` and `"down"`. Each row of the matrix corresponds to a bin, while each column corresponds to a library in `files`. Each entry of the matrix stores the total count of read pairs to upstream or downstream bins.

The total up- and downstream counts can be used to compute a directionality statistic, e.g., as defined by Dixon et al, or by computing the log-fold change between fields. Alternatively, it can be used to identify differential domains - see the user's guide for more details.

### Value

A RangedSummarizedExperiment object with one row for each bin in the genome. It contains two integer matrices named `"up"` and `"down"`, containing the counts to upstream and downstream bins respectively.

### Author(s)

Aaron Lun

### References

Dixon JR et al. (2012). Topological domains in mammalian genomes identified by analysis of chromatin interactions. Nature 485:376-380.

### See Also

`squareCounts`

### Examples

    hic.file <- system.file("exdata", "hic_sort.bam", package="diffHic")
    cuts <- readRDS(system.file("exdata", "cuts.rds", package="diffHic"))
    param <- pairParam(fragments=cuts)

    # Setting up the parameters
    fout <- tempfile(fileext=".h5")
    invisible(preparePairs(hic.file, param, file=fout))

    # Not really that informative; see user's guide.
    out <- domainDirections(fout, param, width=10)
    out

    # Calculating directionality log-FC with a large prior.
    up.counts <- assay(out, "up")
    down.counts <- assay(out, "down")
    dir.logFC <- log2((up.counts+10)/(down.counts+10))
    dir.logFC

    # Calculating directionality index with Dixon's method.
    dixon.stat <- sign(up.counts-down.counts)*2*(
        (up.counts-down.counts)/(up.counts+down.counts))^2
    dixon.stat

[Package diffHic version 1.19.0 Index]