Package: TAPseq
Type: Package
Title: Targeted scRNA-seq primer design for TAP-seq
Version: 0.99.6
Authors@R: c(
  person("Andreas", "Gschwind", email = "andreas.gschwind@stanford.edu",
         role = c("aut", "cre"), comment = c(ORCID = "0000-0002-0769-6907")),
  person("Lars", "Velten", email = "lars.velten@crg.eu", role = "aut",
         comment = c(ORCID = "0000-0002-1233-5874")),
  person("Lars", "Steinmetz", role = "aut"))
Description: Design primers for targeted single-cell RNA-seq used by TAP-seq. Create sequence
  templates for target gene panels and design gene-specific primers using Primer3. Potential
  off-targets can be estimated with BLAST. Requires working installations of Primer3 and BLASTn.
URL: https://github.com/argschwind/TAPseq
BugRepots: https://github.com/argschwind/TAPseq/issues
biocViews: SingleCell, Sequencing, Technology, CRISPR, PooledScreens
License: MIT + file LICENSE
Encoding: UTF-8
RoxygenNote: 7.0.2
VignetteBuilder: knitr
Depends: R (>= 4.0)
Imports: methods, GenomicAlignments, GenomicRanges, IRanges,
        BiocGenerics, S4Vectors (>= 0.20.1), GenomeInfoDb, BSgenome,
        GenomicFeatures, Biostrings, dplyr, tidyr, BiocParallel
Suggests: testthat, BSgenome.Hsapiens.UCSC.hg38, knitr, rmarkdown,
        ggplot2, Seurat, glmnet, cowplot, Matrix, rtracklayer
SystemRequirements: Primer3 (>= 2.5.0), BLAST+ (>=2.6.0)
git_url: https://git.bioconductor.org/packages/TAPseq
git_branch: master
git_last_commit: 50a16a4
git_last_commit_date: 2020-03-17
Date/Publication: 2020-03-24
NeedsCompilation: no
Packaged: 2020-03-25 07:46:41 UTC; biocbuild
Author: Andreas Gschwind [aut, cre] (<https://orcid.org/0000-0002-0769-6907>),
  Lars Velten [aut] (<https://orcid.org/0000-0002-1233-5874>),
  Lars Steinmetz [aut]
Maintainer: Andreas Gschwind <andreas.gschwind@stanford.edu>
Built: R 4.0.0; ; 2020-03-25 16:49:41 UTC; windows
