trimTails {ShortRead} | R Documentation

## Trim ends of reads based on nucleotides or qualities

### Description

These generic functions remove leading or trailing nucleotides or qualities. `trimTails` and `trimTailw` remove low-quality reads from the right end using a sliding window (`trimTailw`) or a tally of (successive) nucleotides falling at or below a quality threshold (`trimTails`). `trimEnds` takes an alphabet of characters to remove from either left or right end.

### Usage

    ## S4 methods for 'ShortReadQ', 'FastqQuality', or 'SFastqQuality'
    trimTailw(object, k, a, halfwidth, ..., ranges=FALSE)
    trimTails(object, k, a, successive=FALSE, ..., ranges=FALSE)
    trimEnds(object, a, left=TRUE, right=TRUE, relation=c("<=", "=="),
        ..., ranges=FALSE)

    ## S4 method for signature 'BStringSet'
    trimTailw(object, k, a, halfwidth, ..., alphabet, ranges=FALSE)
    ## S4 method for signature 'BStringSet'
    trimTails(object, k, a, successive=FALSE, ...,
        alphabet, ranges=FALSE)

    ## S4 method for signature 'character'
    trimTailw(object, k, a, halfwidth, ..., destinations, ranges=FALSE)
    ## S4 method for signature 'character'
    trimTails(object, k, a, successive=FALSE, ..., destinations, ranges=FALSE)
    ## S4 method for signature 'character'
    trimEnds(object, a, left=TRUE, right=TRUE, relation=c("<=", "=="),
        ..., destinations, ranges=FALSE)

### Arguments

`object` | An object (e.g., `ShortReadQ` and derived classes; see below to discover these methods) or character vector of fastq file(s) to be trimmed.
`k` | `integer(1)` describing the number of failing letters required to trigger trimming.
`a` | For `trimTails` and `trimTailw`, a `character(1)` with `nchar(a) == 1L` giving the letter at or below which a nucleotide is marked as failing. For `trimEnds` a `character()` with all `nchar() == 1L` giving the letter at or below which a nucleotide or quality scores marked for removal.
`halfwidth` | The half width (cycles before or after the current; e.g., a half-width of 5 would span 5 + 1 + 5 cycles) in which qualities are assessed.
`successive` | `logical(1)` indicating whether failures can occur anywhere in the sequence, or must be successive. If `successive=FALSE`, then the k'th failed letter and subsequent are removed. If `successive=TRUE`, the first succession of k failed and subsequent letters are removed.
`left, right` | `logical(1)` indicating whether trimming is from the left or right ends.
`relation` | `character(1)` selected from the argument values, i.e., “<=” or “==” indicating whether all letters at or below the `alphabet(object)` are to be removed, or only exact matches.
`...` | Additional arguments, perhaps used by methods.
`destinations` | For `object` of type `character()`, an equal-length vector of destination files. Files must not already exist.
`alphabet` | `character()` (ordered low to high) letters on which quality scale is measured. Usually supplied internally (user does not need to specify). If missing, then set to ASCII characters 0-127.
`ranges` | `logical(1)` indicating whether the trimmed object, or only the ranges satisfying the trimming condition, be returned.

### Details

`trimTailw` starts at the left-most nucleotide, tabulating the number of cycles in a window of `2 * halfwidth + 1` surrounding the current nucleotide with quality scores that fall at or below `a`. The read is trimmed at the first nucleotide for which this number `>= k`. The quality of the first or last nucleotide is used to represent portions of the window that extend beyond the sequence.

`trimTails` starts at the left-most nucleotide and accumulates cycles for which the quality score is at or below `a`. The read is trimmed at the first location where this number `>= k`. With `successive=TRUE`, failing qualities must occur in strict succession.

`trimEnds` examines the `left`, `right`, or both ends of `object`, marking for removal letters that correspond to `a` and `relation`. The `trimEnds,ShortReadQ-method` trims based on quality.

`ShortReadQ` methods operate on quality scores; use `sread()` and the `ranges` argument to trim based on nucleotide (see examples).

`character` methods transform one or several fastq files to new fastq files, applying trim operations based on quality scores; use `filterFastq` with your own `filter` argument to filter on nucleotides.

### Value

An instance of `class(object)` trimmed to contain only those nucleotides satisfying the trim criterion or, if `ranges=TRUE` an `IRanges` instance defining the ranges that would trim `object`.

### Note

The `trim*` functions use OpenMP threads (when available) during creation of the return value. This may sometimes create problems when a process is already running on multiple threads, e.g., with an error message like

        libgomp: Thread creation failed: Resource temporarily unavailable

A solution is to precede problematic code with the following code snippet, to disable threading

        nthreads <- .Call(ShortRead:::.set_omp_threads, 1L)
        on.exit(.Call(ShortRead:::.set_omp_threads, nthreads))

### Author(s)

Martin Morgan <mtmorgan@fhcrc.org>

### Examples

    showMethods(trimTails)

    sp <- SolexaPath(system.file('extdata', package='ShortRead'))
    rfq <- readFastq(analysisPath(sp), pattern="s_1_sequence.txt")

    ## remove leading / trailing quality scores <= 'I'
    trimEnds(rfq, "I")
    ## remove leading / trailing 'N's
    rng <- trimEnds(sread(rfq), "N", relation="==", ranges=TRUE)
    narrow(rfq, start(rng), end(rng))
    ## remove leading / trailing 'G's or 'C's
    trimEnds(rfq, c("G", "C"), relation="==")

[Package ShortRead version 1.45.4 Index]