subtractBackground {RNAither} | R Documentation

## Background substraction

### Description

Substracts a specified background value from the intensity values.

### Usage

    subtractBackground(header, dataset, listOfArgs)

### Arguments

`header` | the header of a dataset file generated with `generateDatasetFile`
`dataset` | an R data frame generated with `generateDatasetFile`
`listOfArgs` | a list containing: - a character string specifying the column whose values will be used for background substraction - a character string specifying the column whose values will be used as background

### Value

A list containing:

`header` | The new header (with an added entry about the normalization procedure in the comments)
`dataset` | The new dataset with normalized values. The old values are saved in an extra column of the dataset with the suffix ".old"

### Examples

    data(exampleHeader, package="RNAither")
    data(exampleDataset, package="RNAither")

    normres <- subtractBackground(header, dataset, list("SigIntensity", "Background"))
    newheader <- normres[[1]]
    newdataset <- normres[[2]]

[Package RNAither version 2.35.0 Index]