ZPRIMEQualControl {RNAither} | R Documentation

## Computing the Z' factor

### Description

Computes the Z' factor per plate for a complete dataset file and plots the results.

### Usage

    ZPRIMEQualControl(header, data, channel, plotTitle, showPlot)

### Arguments

`header` | the header of a dataset file generated with `generateDatasetFile`
`data` | an R data frame generated with `generateDatasetFile`
`channel` | a character string specifying the name of the column containing the values for computing the Z' factor, e.g. `"SigIntensity"`
`plotTitle` | the plot title
`showPlot` | 0 or 1. 1 will open one or several plot windows in the R GUI, 0 will only save the plot(s) without opening windows.

### Value

Returns the Z' values in the shell for each plate and saves them in a text file. The name of the text file will be the concatenation of the experiment name specified in the header and the character string "Z'Scores.txt".

Shows a plot of the Z' factor values and saves it as a png and a pdf file under the experiment name specified in the header concatenated with the function argument `plotTitle`.

The function returns a list containing:

`plotName` | the plot name
`ZPrimeTabelle` | table containing the Z' values

### References

J. Zhang, T. Chung, and K. Oldenburg. A simple statistical parameter for use in evaluation and validation of high throughput screening assays. J Biomol Screen, 4:67-73, 1999.

### Examples

    data(exampleHeader, package="RNAither")
    data(exampleDataset, package="RNAither")

    res <- ZPRIMEQualControl(header, dataset, "SigIntensity", "Z' factors per plate", 1)
    zprime_plot <- res[[1]]
    zprime_table <- res[[2]]

[Package RNAither version 2.35.0 Index]