combine.networks {Pigengene} | R Documentation

## Combines two or more networks

### Description

Takes as input two or more adjacency matrices, and the corresponding contributions. Computes a combined network (weighted graph) in which the weight on an edge between two nodes is an average of the weights on the same edge in the input networks.

### Usage

    combine.networks(nets, contributions, outPath, midfix="",
        powerVector=1:20, verbose=1, RsquaredCut=0.75, minModuleSize=5,
        doRemoveTOM=TRUE, datExpr, doReturNetworks=FALSE, doSave=FALSE)

### Arguments

`nets` | A list of adjacency matrices (networks), which can be generated using e.g., the `WGCNA::adjacency` function. Rows and columns must be named.
`contributions` | A numeric vector with the same length as nets. In computing the average weight on each edge in the combined network, first the edge weights from individual networks are multiplied by their corresponding contributions, then the result will be divided by the sum of weights of all networks containing this edge.
`outPath` | A string to the path where plots and results will be saved.
`midfix` | An optional string used in the output file names.
`powerVector` | A numeric vector of power values that are tried to find the best one. See `WGCNA::pickSoftThreshold` documentation.
`verbose` | The integer level of verbosity. 0 means silent and higher values produce more details of computation.
`RsquaredCut` | A threshold in the range [0,1] used to estimate the power. A higher value can increase power. For technical use only. See `pickSoftThreshold` for more details.
`minModuleSize` | The value that controls the minimum number of genes per module.
`doRemoveTOM` | A boolean determining the big TOM file must remove or not.
`datExpr` | The expression matrix that `WGCNA::blockwiseModules` uses for fine-tuning and removing genes from modules. This is not an ideal behavior by WGCNA.
`doReturNetworks` | A boolean value to determine whether to return `Network`, which is relatively a big matrix (typically GBs). Set to `FALSE` not to waste memory.
`doSave` | A boolean value to determine whether the whole output of this function (typically 1-2 GBs) should be saved as `combinedNetwork`. Set to `FALSE` not to waste disk space.

### Value

A list with following components

`call` | The command that created the results
`midfix` | The input argument
`Network` | The adjacency matrix of the combined network
`denominators` | A matrix, each cell of which is the sum of weights of all networks contributing to the edge corresponding to that cell
`power` | The power (beta) value used for the combined network
`fits` | The fit indices calculated for the combined network
`net` | The output of `WGCNA::blockwiseModules` containing the module information in its `colors` field
`modules` | The output of `WGCNA::blockwiseModules`
`combinedNetworkFile` | The path to the saved file containing `combinedNetwork`

### Note

If the networks have different node sets, the combined network will be computed on the union of nodes.

### See Also

`WGCNA::blockwiseModules`, `WGCNA::TOMsimilarity`, and `WGCNA::pickSoftThreshold.fromSimilarity`

### Examples

    data(aml)
    data(mds)
    nets <- list()
    ## Make the coexpression networks:
    nets[["aml"]] <- abs(stats::cor(aml[,1:200]))
    nets[["mds"]] <- abs(stats::cor(mds[,1:200]))
    ## Combine them:
    combined <- combine.networks(nets=nets, contributions=c(nrow(aml), nrow(mds)),
                     outPath=".", datExpr=rbind(aml, mds)[,1:200])
    print(table(combined$modules))

[Package Pigengene version 1.13.14 Index]