parallelPCA {PCAtools} | R Documentation

## Horn's parallel analysis

### Description

Perform Horn's parallel analysis to choose the number of principal components to retain.

### Usage

    parallelPCA(mat, max.rank=100, ..., niters=50, threshold=0.1,
      transposed=FALSE, BSPARAM=ExactParam(), BPPARAM=SerialParam())

### Arguments

`mat` | A numeric matrix where rows correspond to variables and columns correspond to samples.
`max.rank` | Integer scalar specifying the maximum number of PCs to retain.
`...` | Further arguments to pass to `pca`.
`niters` | Integer scalar specifying the number of iterations to use for the parallel analysis.
`threshold` | Numeric scalar representing the “p-value” threshold above which PCs are to be ignored.
`transposed` | Logical scalar indicating whether `mat` is transposed, i.e., rows are samples and columns are variables.
`BSPARAM` | A BiocSingularParam object specifying the algorithm to use for PCA.
`BPPARAM` | A BiocParallelParam object specifying how the iterations should be paralellized.

### Details

Horn's parallel analysis involves shuffling observations within each row of `x` to create a permuted matrix. PCA is performed on the permuted matrix to obtain the percentage of variance explained under a random null hypothesis. This is repeated over several iterations to obtain a distribution of curves on the scree plot.

For each PC, the “p-value” (for want of a better word) is defined as the proportion of iterations where the variance explained at that PC is greater than that observed with the original matrix. The number of PCs to retain is defined as the last PC where the p-value is below `threshold`. This aims to retain all PCs that explain “significantly” more variance than expected by chance.

This function can be sped up by specifying `BSPARAM=IrlbaParam()` or similar, to use approximate strategies for performing the PCA. Another option is to set `BPPARAM` to perform the iterations in parallel.

### Value

A list is returned, containing:

- `original`, the output from running `pca` on `mat` with the specified arguments.

- `permuted`, a matrix of variance explained from randomly permuted matrices. Each column corresponds to a single permutated matrix, while each row corresponds to successive principal components.

- `n`, the estimated number of principal components to retain.

### Author(s)

Aaron Lun

### References

Horn JL. (1965). A rationale and test for the number of factors in factor analysis. Psychometrika 30(2), 179-185.

Buja A and Eyuboglu N (1992). Remarks on Parallel Analysis. Multivariate Behav. Res. 27, 509-40.

### Examples

    # Mocking up some data.
    ngenes <- 1000
    means <- 2^runif(ngenes, 6, 10)
    dispersions <- 10/means + 0.2
    nsamples <- 50
    counts <- matrix(rnbinom(ngenes*nsamples, mu=means,
        size=1/dispersions), ncol=nsamples)

    # Choosing the number of PCs
    lcounts <- log2(counts + 1)
    output <- parallelPCA(lcounts)
    output$n

[Package PCAtools version 1.99.6 Index]