bedtools_jaccard {HelloRanges} | R Documentation

## bedtools_jaccard

### Description

Compare two sets of genomic regions using the Jaccard statistic, defined as the total width of the intersection, divided by the total width of the union.

### Usage

    bedtools_jaccard(cmd = "--help")
    R_bedtools_jaccard(a, b, f = 1e-09, F = 1e-09, r = FALSE, e = FALSE,
                       s = FALSE, S = FALSE, split = FALSE)
    do_bedtools_jaccard(a, b, f = 1e-09, F = 1e-09, r = FALSE, e = FALSE,
                        s = FALSE, S = FALSE, split = FALSE)

### Arguments

`cmd` | String of bedtools command line arguments, as they would be entered at the shell. There are a few incompatibilities between the docopt parser and the bedtools style. See argument parsing.
`a` | Path to a BAM/BED/GFF/VCF/etc file, a BED stream, a file object, or a ranged data structure, such as a GRanges. Use `"stdin"` for input from another process (presumably while running via `Rscript`). For streaming from a subprocess, prefix the command string with “<”, e.g., `"<grep foo file.bed"`. Any streamed data is assumed to be in BED format.
`b` | Like `a`, except supports multiple datasets, either as a vector/list or a comma-separated string. Also supports file glob patterns, i.e., strings containing the wildcard, “*”.
`f` | Minimum overlap required as a fraction of `a` [default: any overlap].
`F` | Minimum overlap required as a fraction of `b` [default: any overlap].
`r` | Require that the fraction of overlap be reciprocal for `a` and `b`. In other words, if `f` is 0.90 and `r` is `TRUE`, this requires that `b` overlap at least 90% of `a` and that `a` also overlaps at least 90% of `b`.
`e` | Require that the minimum fraction be satisfied for `a` OR `b`. In other words, if `e` is `TRUE` with `f=0.90` and `F=0.10` this requires that either 90% of `a` is covered OR 10% of `b` is covered. If `FALSE`, both fractions would have to be satisfied.
`s` | Require same strandedness. That is, find the jaccard feature in `b` that overlaps `a` on the same strand. By default, overlaps are reported without respect to strand. Note that this is the exact opposite of Bioconductor behavior.
`S` | Require opposite strandedness. That is, find the jaccard feature in `b` that overlaps `a` on the opposite strand. By default, overlaps are reported without respect to strand.
`split` | Treat split BAM (i.e., having an ‘N’ CIGAR operation) or BED12 entries as compound ranges with gaps, i.e., as GRangesList objects.

### Details

As with all commands, there are three interfaces to the `jaccard` command:

`bedtools_jaccard`

  Parses the bedtools command line and compiles it to the equivalent R code.

`R_bedtools_jaccard`

  Accepts R arguments corresponding to the command line arguments and compiles the equivalent R code.

`do_bedtools_jaccard`

  Evaluates the result of `R_bedtools_jaccard`. Recommended only for demonstration and testing. It is best to integrate the compiled code into an R script, after studying it.

This is mostly just `intersect` and `union`, except when fractional overlap restrictions are involved.

### Value

A language object containing the compiled R code, evaluating to a a DataFrame with four columns:

`intersection` | total width of intersection
`union` | total width of union
`jaccard` | the jaccard statistic
`n_intersections` | the number of ranges representing the intersection

### Author(s)

Michael Lawrence

### References

http://bedtools.readthedocs.io/en/latest/content/tools/jaccard.html

### See Also

setops-methods for set operations including intersect and union.

### Examples

    ## Not run:
    setwd(system.file("unitTests", "data", "jaccard", package="HelloRanges"))

    ## End(Not run)

    ## basic
    bedtools_jaccard("-a a.bed -b a.bed")
    ## excluding the gaps in compound ranges
    bedtools_jaccard("-a three_blocks_match.bed -b e.bed -split")
    ## strand and fractional overlap restriction
    bedtools_jaccard("-a aMixedStrands.bed -b bMixedStrands.bed -s -f 0.8")

[Package HelloRanges version 1.13.0 Index]