uniqueREs {CRISPRseek} | R Documentation

## Output restriction enzymes that recognize only the gRNA cleavage sites

### Description

For each identified gRNA, output restriction enzymes that recognize only the gRNA cleavage sites.

### Usage

    uniqueREs(REcutDetails, summary, offTargets, scanUpstream = 100,
       scanDownstream = 100, BSgenomeName)

### Arguments

`REcutDetails` | REcutDetails stored in the REcutDetails.xls
`summary` | summary stored in the summary.xls
`offTargets` | offTargets stored in the offTargets.xls
`scanUpstream` | upstream offset from the gRNA start, default 100
`scanDownstream` | downstream offset from the gRNA end, default 100
`BSgenomeName` | BSgenome object. Please refer to available.genomes in BSgenome package. For example, BSgenome.Hsapiens.UCSC.hg19 for hg19, BSgenome.Mmusculus.UCSC.mm10 for mm10, BSgenome.Celegans.UCSC.ce6 for ce6, BSgenome.Rnorvegicus.UCSC.rn5 for rn5, BSgenome.Drerio.UCSC.danRer7 for Zv9, and BSgenome.Dmelanogaster.UCSC.dm3 for dm3

### Value

returns the RE sites that recognize only the gRNA cleavage sites for each gRNA.

### Author(s)

Lihua Julie Zhu

### Examples

        library("BSgenome.Hsapiens.UCSC.hg19")
        load(system.file("extdata", "ForTestinguniqueREs.RData",
                package = "CRISPRseek"))
        uniqueREs(results$REcutDetails, results$summary, results$offtarget,
    	scanUpstream = 50,
            scanDownstream = 50, BSgenomeName = Hsapiens)

[Package CRISPRseek version 1.27.5 Index]