orderCellsMonocle {phemd} | R Documentation

## Compute Monocle2 cell state and pseudotime assignments

### Description

Takes as input a Phemd object with Monocle2 object and returns updated object with Monocle2 object containing cell state and pseudotime assignments

### Usage

    orderCellsMonocle(obj, ...)

### Arguments

`obj` | 'Phemd' object containing Monocle2 object initialized using embedCells
`...` | Additional parameters to be passed into `orderCells` function

### Details

Wrapper function for `orderCells` in Monocle 2 package. `embedCells` needs to be called before calling this function.

### Value

Same as input 'Phemd' object with updated cell-state embedding object containing cell state assignments

### Examples

    my_phemdObj <- createDataObj(all_expn_data, all_genes, as.character(snames_data))
    my_phemdObj_lg <- removeTinySamples(my_phemdObj, 10)
    my_phemdObj_lg <- aggregateSamples(my_phemdObj_lg, max_cells=1000)
    my_phemdObj_monocle <- embedCells(my_phemdObj_lg, cell_model='monocle2', data_model='gaussianff', sigma=0.02, maxIter=2)
    my_phemdObj_monocle <- orderCellsMonocle(my_phemdObj_monocle)

[Package phemd version 1.4.0 Index]