specUnmix {flowSpecs} | R Documentation

## Spectral unmixing of cytometry files

### Description

This function performs the central task of spectral unmixing, to convert the raw photon detector input to "biological" proxy-signals.

### Usage

    specUnmix(flowObj, specMat)

### Arguments

`flowObj` | The fcs object to be filtered. Both flowFrames and flowSets are accepted.
`specMat` | This is a matrix generated by the secMatCalc function, possibly with edited row names.

### Value

The unmixed data. It will be returned in the format it was imported as.

### Examples

    # Load uncompensated data
    data(fullPanel)

    # Load the spectral unmixing matrix generated with controls from the same
    # experiment. These can be generated using the specMatCalc function.
    data(specMat)

    # And now, just run the function
    fullPanelUnmix <- specUnmix(fullPanel, specMat)

[Package flowSpecs version 1.2.0 Index]