dyebias-package {dyebias} | R Documentation

## The GASSCO method for correcting for slide-dependent gene-specific dye bias

### Description

Many two-colour hybridizations suffer from a dye bias that is both gene-specific and slide-specific. The former depends on the content of the nucleotide used for labeling; the latter depends on the labeling percentage. The slide-dependency was hitherto not recognized, and made addressing the artefact impossible. Given a reasonable number of dye-swapped pairs of hybridizations, or of same vs. same hybridizations, both the gene- and slide-biases can be estimated and corrected using the GASSCO method (Margaritis et al., Mol. Sys. Biol. 5:266 (2009), doi:10.1038/msb.2009.21)

### Details

Package: | dyebias
Type: | Package
Version: | 1.7.1
Date: | 26 May 2010
Licence: | GPL-3

### Author(s)

Philip Lijnzaad and Thanasis Margaritis
Philip Lijnzaad <p.lijnzaad@umcutrecht.nl> (Maintainer).

### See Also

`dyebias.application.subset`, `dyebias.apply.correction`, `dyebias.boxplot`, `dyebias.estimate.iGSDBs`, `dyebias.monotonicity`, `dyebias.monotonicityplot`, `dyebias.rgplot`, `dyebias.trendplot`.

[Package dyebias version 1.48.0 Index]