getFeatureBoundaryCoverage {RCAS} | R Documentation

## getFeatureBoundaryCoverage

### Description

This function extracts the flanking regions of 5' and 3' boundaries of a given set of genomic features and computes the per-base coverage of query regions across these boundaries.

### Usage

    getFeatureBoundaryCoverage(
      queryRegions,
      featureCoords,
      flankSize = 500,
      boundaryType,
      sampleN = 0
    )

### Arguments

`queryRegions` | GRanges object imported from a BED file using `importBed` function
`featureCoords` | GRanges object containing the target feature coordinates
`flankSize` | Positive integer that determines the number of base pairs to extract around a given genomic feature boundary
`boundaryType` | (Options: fiveprime or threeprime). Denotes which side of the feature's boundary is to be profiled.
`sampleN` | A positive integer value less than the total number of featuer coordinates that determines whether the target feature coordinates should be randomly downsampled. If set to 0, no downsampling will happen. If

### Value

a data frame containin three columns. 1. fivePrime: Coverage at 5' end of features 2. threePrime: Coverage at 3' end of features; 3. bases: distance (in bp) to the boundary

### Examples

    data(queryRegions)
    data(gff)
    txdb <- GenomicFeatures::makeTxDbFromGRanges(gff)
    transcriptCoords <- GenomicFeatures::transcripts(txdb)
    transcriptEndCoverage <- getFeatureBoundaryCoverage (
                                         queryRegions = queryRegions,
                                        featureCoords = transcriptCoords,
                                        flankSize = 100,
                                        boundaryType = 'threeprime',
                                        sampleN = 1000)

[Package RCAS version 1.14.0 Index]