SequenceTrack-class {Gviz} | R Documentation

## SequenceTrack class and methods

### Description

A track class to represent genomic sequences. The three child classes `SequenceDNAStringSetTrack`, `SequenceRNAStringSetTrack` and `SequenceBSgenomeTrack` do most of the work, however in practise they are of no particular relevance to the user.

### Usage

    SequenceTrack(sequence, chromosome, genome, name="SequenceTrack",
    importFunction, stream=FALSE, ...)

    RNASequenceTrack(sequence, chromosome, genome, name="SequenceTrack",
    importFunction, stream=FALSE, ...)

### Arguments

`sequence` | A meta argument to handle the different input types, making the construction of a `SequenceTrack` as flexible as possible. The different input options for `sequence` are: An object of class `DNAStringSet`. The individual `DNAString`s are considered to be the different chromosome sequences. An object of class `BSgenome`. The `Gviz` package tries to follow the `BSgenome` philosophy in that the respective chromosome sequences are only realized once they are first accessed. A `character` scalar: in this case the value of the `sequence` argument is considered to be a file path to an annotation file on disk. A range of file types are supported by the `Gviz` package as identified by the file extension. See the `importFunction` documentation below for further details.
`chromosome` | The currently active chromosome of the track. A valid UCSC chromosome identifier if `options(ucscChromosomeNames=TRUE)`. Please note that in this case only syntactic checking takes place, i.e., the argument value needs to be an integer, numeric character or a character of the form `chrx`, where `x` may be any possible string. The user has to make sure that sequences for the respective chromosomes are indeed part of the object. If not provided here, the constructor will set it to the first available sequence. Please note that by definition all objects in the `Gviz` package can only have a single active chromosome at a time (although internally the information for more than one chromosome may be present), and the user has to call the `chromosome<-` replacement method in order to change to a different active chromosome.
`genome` | The genome on which the track's ranges are defined. Usually this is a valid UCSC genome identifier, however this is not being formally checked at this point. For a `SequenceBSgenomeTrack` object, the genome information is extracted from the input `BSgenome` package. For a `DNAStringSet` it has too be provided or the constructor will fall back to the default value of `NA`.
`name` | Character scalar of the track's name used in the title panel when plotting.
`importFunction` | A user-defined function to be used to import the sequence data from a file. This only applies when the `sequence` argument is a character string with the path to the input data file. The function needs to accept an argument `file` containing the file path and has to return a proper `DNAStringSet` object with the sequence information per chromosome. A set of default import functions is already implemented in the package for a number of different file types, and one of these defaults will be picked automatically based on the extension of the input file name. If the extension can not be mapped to any of the existing import function, an error is raised asking for a user-defined import function. Currently the following file types can be imported with the default functions: `fa/fasta` and `2bit`. Both file types support indexing by genomic coordinates, and it makes sense to only load the part of the file that is needed for plotting. To this end, the `Gviz` package defines the derived `ReferenceSequenceTrack` class, which supports streaming data from the file system. The user typically does not have to deal with this distinction but may rely on the constructor function to make the right choice as long as the default import functions are used. However, once a user-defined import function has been provided and if this function adds support for indexed files, you will have to make the constructor aware of this fact by setting the `stream` argument to `TRUE`. Please note that in this case the import function needs to accept a second mandatory argument `selection` which is a `GRanges` object containing the dimensions of the plotted genomic range. As before, the function has to return an appropriate `DNAStringSet` object.
`stream` | A logical flag indicating that the user-provided import function can deal with indexed files and knows how to process the additional `selection` argument when accessing the data on disk. This causes the constructor to return a `ReferenceSequenceTrack` object which will grab the necessary data on the fly during each plotting operation.
`...` | Additional items which will all be interpreted as further display parameters. See `settings` and the "Display Parameters" section below for details.

### Value

The return value of the constructor function is a new object of class `SequenceDNAStringSetTrack`, `SequenceBSgenomeTrack` ore `ReferenceSequenceTrack`, depending on the constructor arguments. Typically the user will not have to be troubled with this distinction and can rely on the constructor to make the right choice.

### Objects from the class

Objects can be created using the constructor function `SequenceTrack`.

### details

Depending on the available space the class will use different options to plot a sequence. If single letters can be accomodated without overplotting those will be show. Otherwise, colored boxes will be used to indicate letters, and if there is not enough horizontal room to show those, a simple line will indicate presence of a sequence. The `min.width` and `fontsize` display parameters directly control this behaviour. Each of the five possible nucleotides (G, A, T, C, and N) will be endoded in a separate color. As default we use the colors suggested in the `biovizBase` package, but a user is free to set their own color scheme by providing a named character vector with color as display parameter `fontcolor`, with names equal to the five possible bases.

### Slots

`chromosome`:

  Object of class `"character"`, the chromosome on which the track is defined. There can only be a single chromosome for one track. Throughout the package, chromosome name have to be entered either as a single integer scalar or as a character scalar of the form `chrXYZ`, where XYZ may be an arbitrary character string.

`genome`:

  Object of class `"character"`, the genome for which the track is defined. This should be a valid UCSC genome identifier, however this may not always be formally checked upon object instantiation.

`dp`:

  Object of class `DisplayPars`, inherited from class `GdObject`.

`name`:

  Object of class `"character"`, inherited from class `GdObject`

`imageMap`:

  Object of class `ImageMap`, inherited from class `GdObject`

### Extends

Class `"GdObject"`, directly.

### Methods

In the following code chunks, `obj` is considered to be an object inheriting from class `SequenceTrack`.

Exported in the name space:

chromosome

  `signature(GdObject="SequenceTrack")`: return the chromosome for which the track is defined.

  Usage:

  `chromosome(GdObject)`

  Examples:

    `chromosome(obj)`

chromosome<-

  `signature(GdObject="SequenceTrack")`: replace the value of the track's chromosome. This has to be a valid UCSC chromosome identifier or an integer or character scalar that can be reasonably coerced into one.

  Usage:

  `chromosome<-(GdObject, value)`

  Additional Arguments:

    `value`: replacement value.

  Examples:

    `chromosome(obj) <- "chr12"`

genome

  `signature(x="SequenceTrack")`: return the track's genome.

  Usage:

  `genome(x)`

  Examples:

    `genome(obj)`

genome<-

  `signature(x="SequenceTrack")`: set the track's genome. Usually this has to be a valid UCSC identifier, however this is not formally enforced here.

  Usage:

  `genome<-(x, value)`

  Additional Arguments:

    `value`: replacement value.

  Examples:

    `genome(obj) <- "mm9"`

length

  `signature(x="SequenceTrack")`: return the number of nucleotides in the track's sequence.

  Usage:

  `length(x)`

  Examples:

    `length(obj)`

seqnames

  `signature(x="SequenceTrack")`: return the names (i.e., the chromosome) of the sequences contained in the object.

  Usage:

  `values(x)`

  Examples:

    `seqnames(obj)`

subseq

  `signature(x="SequenceTrack")`: Extract a sub-sequence from the track.

  Usage:

  `subseq(x, start=NA, end=NA, width=NA)`

  Additional Arguments:

    `start`: the start coordinate for the sub-sequence.

    `end`: the end coordinate for the sub-sequence.

    `width`: the width of the sub-sequence.

  Examples:

    `subseq(obj, 1, 10)`

Internal methods:

initialize

  `signature(.Object="SequenceTrack")`: initialize the object.

Inherited methods:

displayPars

  `signature(x="SequenceTrack", name="character")`: list the value of the display parameter `name`. See `settings` for details on display parameters and customization.

  Usage:

  `displayPars(x, name)`

  Examples:

    `displayPars(obj, "col")`

displayPars

  `signature(x="SequenceTrack", name="missing")`: list the value of all available display parameters. See `settings` for details on display parameters and customization.

  Examples:

    `displayPars(obj)`

getPar

  `signature(x="SequenceTrack", name="character")`: alias for the `displayPars` method. See `settings` for details on display parameters and customization.

  Usage:

  `getPar(x, name)`

  Examples:

    `getPar(obj, "col")`

getPar

  `signature(x="SequenceTrack", name="missing")`: alias for the `displayPars` method. See `settings` for details on display parameters and customization.

  Examples:

    `getPar(obj)`

displayPars<-

  `signature(x="SequenceTrack", value="list")`: set display parameters using the values of the named list in `value`. See `settings` for details on display parameters and customization.

  Usage:

  `displayPars<-(x, value)`

  Examples:

    `displayPars(obj) <- list(col="red", lwd=2)`

setPar

  `signature(x="SequenceTrack", value="character")`: set the single display parameter `name` to `value`. Note that display parameters in the `SequenceTrack` class are pass-by-reference, so no re-assignmnet to the symbol `obj` is necessary. See `settings` for details on display parameters and customization.

  Usage:

  `setPar(x, name, value)`

  Additional Arguments:

    `name`: the name of the display parameter to set.

  Examples:

    `setPar(obj, "col", "red")`

setPar

  `signature(x="SequenceTrack", value="list")`: set display parameters by the values of the named list in `value`. Note that display parameters in the `SequenceTrack` class are pass-by-reference, so no re-assignmnet to the symbol `obj` is necessary. See `settings` for details on display parameters and customization.

  Examples:

    `setPar(obj, list(col="red", lwd=2))`

names

  `signature(x="SequenceTrack")`: return the value of the `name` slot.

  Usage:

  `names(x)`

  Examples:

    `names(obj)`

names<-

  `signature(x="SequenceTrack", value="character")`: set the value of the `name` slot.

  Usage:

  `names<-(x, value)`

  Examples:

    `names(obj) <- "foo"`

coords

  `signature(ImageMap="SequenceTrack")`: return the coordinates from the internal image map.

  Usage:

  `coords(ImageMap)`

  Examples:

    `coords(obj)`

tags

  `signature(x="SequenceTrack")`: return the tags from the internal image map.

  Usage:

  `tags(x)`

  Examples:

    `tags(obj)`

drawAxis

  `signature(GdObject="SequenceTrack")`: add a y-axis to the title panel of a track if necessary. Unless overwritten in one of the sub-classes this usualy does not plot anything and returns `NULL`.

  Usage:

  `drawAxis(x, ...)`

  Additional Arguments:

    `...`: all further arguments are ignored.

  Examples:

    `Gviz:::drawAxis(obj)`

drawGrid

  `signature(GdObject="SequenceTrack")`: superpose a grid on top of a track if necessary. Unless overwritten in one of the sub-classes this usualy does not plot anything and returns `NULL`.

  Usage:

  `drawGrid(GdObject, ...)`

  Additional Arguments:

    `...`: additional arguments are ignored.

  Examples:

    `Gviz:::drawGrid(obj)`

### Display Parameters

The following display parameters are set for objects of class `SequenceTrack` upon instantiation, unless one or more of them have already been set by one of the optional sub-class initializers, which always get precedence over these global defaults. See `settings` for details on setting graphical parameters for tracks.

  `add53=FALSE`: Logical scalar. Add a direction indicator.

  `background.title="transparent"`: Character scalar. Make the title panel background transparent by default.

  `col="darkgray"`: Character scalar. The color of the line when no indiviual letters can be plotted due to size limitations.

  `complement=FALSE`: Logical scalar. Plot the sequence complement.

  `fontface=2`: Numeric scalar. The face of the font.

  `fontsize=10`: Numeric scalar. Controls the size of the sequence letters and thus also the level of plotable details.

  `lwd=2`: Numeric scalar. The width of the line when no indiviual letters can be plotted due to size limitations.

  `min.width=2`: Numeric scalar. The minimum width in pixels of the colored boxes that are drawn when no indiviual letters can be plotted due to size limitations. If the horizontal space that a single base occupies is smaller than this value, only a horizontal line is drawn to indicate the presence of a sequence.

  `noLetters=FALSE`: Logical scalar. Always plot colored boxes (or a line) regardles of the available space.

  `showTitle=FALSE`: Logical scalar. Do not show a title panel by default.

  `size=NULL`: Numeric scalar. The size of the track item. Defaults to auto-detect the size based on the other parameter settings.

Additional display parameters are being inherited from the respective parent classes. Note that not all of them may have an effect on the plotting of `SequenceTrack` objects.

  `GdObject`:

    `alpha=1`: Numeric scalar. The transparency for all track items.

    `alpha.title=NULL`: Numeric scalar. The transparency for the title panel.

    `background.legend="transparent"`: Integer or character scalar. The background color for the legend.

    `background.panel="transparent"`: Integer or character scalar. The background color of the content panel.

    `cex=1`: Numeric scalar. The overall font expansion factor for all text and glyphs, unless a more specific definition exists.

    `cex.axis=NULL`: Numeric scalar. The expansion factor for the axis annotation. Defaults to `NULL`, in which case it is automatically determined based on the available space.

    `cex.title=NULL`: Numeric scalar. The expansion factor for the title panel. This effects the fontsize of both the title and the axis, if any. Defaults to `NULL`, which means that the text size is automatically adjusted to the available space.

    `col.axis="white"`: Integer or character scalar. The font and line color for the y axis, if any.

    `col.border.title="white"`: Integer or character scalar. The border color for the title panels.

    `col.frame="lightgray"`: Integer or character scalar. The line color used for the panel frame, if `frame==TRUE`

    `col.grid="#808080"`: Integer or character scalar. Default line color for grid lines, both when `type=="g"` in `DataTrack`s and when display parameter `grid==TRUE`.

    `col.line=NULL`: Integer or character scalar. Default colors for plot lines. Usually the same as the global `col` parameter.

    `col.symbol=NULL`: Integer or character scalar. Default colors for plot symbols. Usually the same as the global `col` parameter.

    `col.title="white"` `(Aliases: fontcolor.title)`: Integer or character scalar. The border color for the title panels

    `collapse=TRUE`: Boolean controlling whether to collapse the content of the track to accomodate the minimum current device resolution. See `collapsing` for details.

    `fill="lightgray"`: Integer or character scalar. Default fill color setting for all plotting elements, unless there is a more specific control defined elsewhere.

    `fontcolor="black"`: Integer or character scalar. The font color for all text, unless a more specific definition exists.

    `fontface.title=2`: Integer or character scalar. The font face for the title panels.

    `fontfamily="sans"`: Integer or character scalar. The font family for all text, unless a more specific definition exists.

    `fontfamily.title="sans"`: Integer or character scalar. The font family for the title panels.

    `frame=FALSE`: Boolean. Draw a frame around the track when plotting.

    `grid=FALSE`: Boolean, switching on/off the plotting of a grid.

    `h=-1`: Integer scalar. Parameter controlling the number of horizontal grid lines, see `panel.grid` for details.

    `lineheight=1`: Numeric scalar. The font line height for all text, unless a more specific definition exists.

    `lty="solid"`: Numeric scalar. Default line type setting for all plotting elements, unless there is a more specific control defined elsewhere.

    `lty.grid="solid"`: Integer or character scalar. Default line type for grid lines, both when `type=="g"` in `DataTrack`s and when display parameter `grid==TRUE`.

    `lwd.border.title=1`: Integer scalar. The border width for the title panels.

    `lwd.grid=1`: Numeric scalar. Default line width for grid lines, both when `type=="g"` in `DataTrack`s and when display parameter `grid==TRUE`.

    `lwd.title=1`: Integer scalar. The border width for the title panels

    `min.distance=1`: Numeric scalar. The minimum pixel distance before collapsing range items, only if `collapse==TRUE`. See `collapsing` for details.

    `min.height=3`: Numeric scalar. The minimum range height in pixels to display. All ranges are expanded to this size in order to avoid rendering issues. See `collapsing` for details.

    `reverseStrand=FALSE`: Logical scalar. Set up the plotting coordinates in 3' -> 5' direction if `TRUE`. This will effectively mirror the plot on the vertical axis.

    `rotation=0`: The rotation angle for all text unless a more specific definiton exists.

    `rotation.title=90` `(Aliases: rotation.title)`: The rotation angle for the text in the title panel. Even though this can be adjusted, the automatic resizing of the title panel will currently not work, so use at own risk.

    `showAxis=TRUE`: Boolean controlling whether to plot a y axis (only applies to track types where axes are implemented).

    `v=-1`: Integer scalar. Parameter controlling the number of vertical grid lines, see `panel.grid` for details.

### Author(s)

Florian Hahne

### See Also

`AnnotationTrack`

`BSgenome`

`DataTrack`

`DisplayPars`

`DNAString`

`DNAStringSet`

`GdObject`

`GeneRegionTrack`

`GRanges`

`ImageMap`

`IRanges`

`collapsing`

`panel.grid`

`plotTracks`

`settings`

### Examples

    ## An empty object
    SequenceTrack()

    ## Construct from DNAStringSet
    library(Biostrings)
    letters <- c("A", "C", "T", "G", "N")
    set.seed(999)
    seqs <- DNAStringSet(c(chr1=paste(sample(letters, 100000, TRUE),
    collapse=""), chr2=paste(sample(letters, 200000, TRUE), collapse="")))
    sTrack <- SequenceTrack(seqs, genome="hg19")
    sTrack

    ## Construct from BSGenome object
    if(require(BSgenome.Hsapiens.UCSC.hg19)){
    sTrack <- SequenceTrack(Hsapiens)
    sTrack
    }

    ## Set active chromosome
    chromosome(sTrack)
    chromosome(sTrack) <- "chr2"
    head(seqnames(sTrack))

    ## Plotting
    ## Sequences
    plotTracks(sTrack, from=199970, to=200000)
    ## Boxes
    plotTracks(sTrack, from=199800, to=200000)
    ## Line
    plotTracks(sTrack, from=1, to=200000)
    ## Force boxes
    plotTracks(sTrack, from=199970, to=200000, noLetters=TRUE)
    ## Direction indicator
    plotTracks(sTrack, from=199970, to=200000, add53=TRUE)
    ## Sequence complement
    plotTracks(sTrack, from=199970, to=200000, add53=TRUE, complement=TRUE)
    ## Colors
    plotTracks(sTrack, from=199970, to=200000, add53=TRUE, fontcolor=c(A=1,
    C=1, G=1, T=1, N=1))

    ## Track names
    names(sTrack)
    names(sTrack) <- "foo"

    ## Accessors
    genome(sTrack)
    genome(sTrack) <- "mm9"
    length(sTrack)

    ## Sequence extraction
    subseq(sTrack, start=100000, width=20)
    ## beyond the stored sequence range
    subseq(sTrack, start=length(sTrack), width=20)

[Package Gviz version 1.32.0 Index]