Package: CRISPRseek
Type: Package
Title: Design of target-specific guide RNAs in CRISPR-Cas9,
        genome-editing systems
Version: 1.4.2
Date: 2014-10-09
Author: Lihua Julie Zhu, Benjamin R. Holmes, Herve Pages, Neil Aronin and Michael Brodsky
Maintainer: Lihua Julie Zhu <julie.zhu@umassmed.edu>
Depends: R (>= 3.0.1), BiocGenerics, Biostrings, BSgenome, seqinr
biocViews: GeneRegulation, SequenceMatching
Suggests: RUnit, BiocStyle, BSgenome.Hsapiens.UCSC.hg19,
        TxDb.Hsapiens.UCSC.hg19.knownGene, org.Hs.eg.db
Description: The package includes functions to find potential guide RNAs for input target sequences, optionally filter guide RNAs without restriction enzyme cut site, or without paired guide RNAs, genome-wide search for off-targets, score, rank, fetch flank sequence and indicate whether the target and off-targets are located in exon region or not. Potential guide RNAs are annotated with total score of the top5 and topN off-targets, detailed topN mismatch sites, restriction enzyme cut sites, and paired guide RNAs. This package leverages Biostrings and BSgenome packages.
License: GPL (>= 2)
LazyLoad: yes
Packaged: 2014-10-21 04:24:23 UTC; biocbuild
Built: R 3.1.1; ; 2014-10-21 07:12:15 UTC; windows
