fluidigmSC_readLinearExp {uSORT} | R Documentation |
An expression reading function which imports expression data from .txt file, and then computes log2 transformed data, reproduced from FluidigmSC package.
fluidigmSC_readLinearExp(exp_file = TRUE, lod = 1)
exp_file |
Input file name in txt format, with rownames of cells and colnames of genes. |
lod |
A value of limit of detection in the unit of TPM/CPM/RPKM. It will be used as the starting value for outlier cell detection and the basis for removing scarce genes. |
A list containing expression_data_raw
(data frame), log2ex_data
(data frame),
and log2ex_avg_data
(data frame).