podcallThresholds {PoDCall} | R Documentation |
Function sets threshold per channel per well and calculates concentrations. Results are returned as a data frame.
podcallThresholds(plateData, nchannels=c(1,2)[2], B=200, Q=9, refWell=1, updateProgress=NULL)
plateData |
List of data frames with amplitude data from a 96 well plate |
nchannels |
Number of channels used in the experiment (default=2) |
B |
Number of permutations for the Likelihood Ratio Test (LRT) (default=200) |
Q |
Parameter for outlier calling (default=9) |
refWell |
reference well to calculate the shift in baseline (default=1) |
updateProgress |
function to update progress bar in shiny app (default=NULL) |
A table with results and metrics, one row per well.
## Path to example data dataPath <- system.file("extdata", "Amplitudes/", package="PoDCall") ## Read in example data dataList <- importAmplitudeData(dataDirectory=dataPath) ## Set thresholds thresholds <- podcallThresholds(plateData=dataList, B=100)