flowFrame-class {flowCore} | R Documentation |
This class represents the data contained in a FCS 3.0 file or similar data structures.
Objects of class flowFrame
can be used to hold arbitrary
data of cell populations, acquired in flow-cytometry.
FCS 3.0 is the Data File Standard for Flow Cytometry, Version FCS3.0. See the vignette of this package for additional information on using the object system for handling of flow-cytometry data.
Objects can be created using
new("flowFrame",
exprs = ...., Object of class matrix
parameters = ...., Object of class AnnotatedDataFrame
description = ...., Object of class list
)
or the function read.FCS
.
exprs
:matrix
containing
the measured intensities. Rows correspond to cells, columns to the
different channels. The colnames
attribute of the matrix is
supposed to hold the names or identifiers for the channels. The
rownames
attribute would usually not be set.parameters
:AnnotatedDataFrame
containing
information about each column of the flowFrame. This will generally
be filled in by read.FCS
or similar functions using data from
the description
slot that describes the parameters.description
:flowFrame
.
The subsetting is applied to the exprs
slot, while the
description
slot is unchanged.AnnotatedDataFrame
flowFrame
object.flowFrame
object.flowSet
object. See filter for details
flowFrame
objects.flowFrame
object according to a filter or
gate.identifier
for details.flowFrame
F. Hahne, B. Ellis, P. Haaland and N. Le Meur