logicleTransform {flowCore} | R Documentation |
Logicle transformation create a subset of link[flowCore]biexponential
logicleTransform(transformationId, w = 0, r = 262144, d = 5, ...)
transformationId |
A name to assign to the transformation. Used by the transform/filter integration routines. |
w |
positive number that corresponds to the width of the negative data range and the range of linearized datain natural log |
r |
top of the scale data value, e.g, 10000 for common 4 decade data or 262144 for a 18 bit data range. |
d |
breath of the display in natural logarithm units. |
... |
additional arguments |
B. Ellis N. LeMeur
Parks D.R., Roederer M., Moore W.A.(2006) A new "Logicle" display method avoids deceptive effects of logarithmic scaling for low signals and compensated data. CytometryA, 96(6):541-51.
data(GvHD) samp <- read.FCS(system.file("extdata", "0877408774.B08", package="flowCore")) samp <- GvHD[[1]] logicle <- logicleTransform(w=2) after <- transform(samp, `FSC-H`=logicle(`FSC-H`))