plot2sets.FCS {rflowcyt}R Documentation

Create a scatterplot to summaryze and compare two series of FCS objects

Description

Create a scatterplot to summaryze and compare 1 paraneter from two series of FCS objects stored in 2 different plates. The points are colored according to their position in the plate (row or column number.)

Usage

plot2sets.FCS(data1,data2,varpos=c(1),FUN,nrow=8,ncol=12,ind=c(1:96),col="row",labeling=TRUE,...)                      

Arguments

data1 a list of fluorescent data from one (or more) FCS object(s) or a cytoset
data2 a list of fluorescent data from one (or more) FCS object(s) or a cytoset
varpos the numerical column variable position of the FCS objects
FUN function to summaryze the distribution of the data, e.g. mean, median, IQR, MODE
col
nrow numeric, number of rows per plate
ncol numeric, number of columns per plate
ind numeric vector, index of the wells to be plotted
labeling
... any other arguments are passed to the plot function

Value

None.

Author(s)

Nolwenn Le Meur

See Also

plot

Examples

  ##Example I:
  ##data(flowcyt.data)

 ##Draw a scatterplot of the median values
 ##of the Foward scatter and the Side scatter parameters
 ##of each FCS file. The files correspond to samples store in a 96 well plate.
 ##plot2sets.FCS(flowcyt.data,varpos=c(1,2),FUN1=median,nrow=8,ncol=10,ind=c(1:80),col="row",pch="*",labeling=FALSE,xlim=c(0,300),ylim=c(0,300),main="FSCmedian vs.SSCmedian by row",xlab="SSC median",ylab="FSC median")

 
 

[Package rflowcyt version 1.4.0 Index]