readFCS {prada}R Documentation

Read an FCS 3.0 file

Description

Read an FCS 3.0 file: Data File Standard for Flow Cytometry, Version FCS3.0

Usage

  readFCS(filename)

Arguments

filename Character of length 1: filename

Details

For specifications of FACS 3.0 see http://www.isac-net.org and the file ../doc/fcs3.html in the doc directory of the package.

The FCS 3.0 has many options that are not yet implemented in this function. It works for the data produced by the FACS machine Calibur from BD Bioscience together with the software Cellquest Pro at the Division of Molecular Genome Analysis at the German Cancer Research Center. If you need extensions, please let me know.

Value

A matrix with column names (one row per cell, one column per measured parameter), and attribute text, a named character vector with keyword-value pairs.

Author(s)

Wolfgang Huber http://www.dkfz.de/abt0840/whuber

See Also

readFCSdata

Examples

sampdat <- readFCS(system.file("extdata", "fas Bcl2 plate323-04-04.A01", package="prada"))
sampdat[1:5,]
dim(sampdat)
attr(sampdat, "text")[3:6]

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