Friendly tidy wrappers for streamlined bulk transcriptional analysis


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Documentation for package ‘tidybulk’ version 0.99.23

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A B C D E F G I K L M N P R S T U X

-- A --

add_attr Add attribute to abject
add_class Add class to abject
add_scaled_counts_bulk.calcNormFactor Calculate the norm factor with calcNormFactor from limma
add_scaled_counts_bulk.get_low_expressed Drop lowly tanscribed genes for TMM normalization
adjust_abundance Adjust transcript abundance for unwanted variation
adjust_abundance-method adjust_abundance
adjust_abundance-method adjust_abundance
adjust_abundance-method adjust_abundance
adjust_abundance-method adjust_abundance
adjust_abundance-method adjust_abundance
aggregate_duplicated_transcripts_bulk Aggregates multiple counts from the same samples (e.g., from isoforms) This function aggregates counts over samples, concatenates other character columns, and averages other numeric columns
aggregate_duplicates Aggregates multiple counts from the same samples (e.g., from isoforms), concatenates other character columns, and averages other numeric columns
aggregate_duplicates-method aggregate_duplicates
aggregate_duplicates-method aggregate_duplicates
aggregate_duplicates-method aggregate_duplicates
aggregate_duplicates-method aggregate_duplicates
aggregate_duplicates-method aggregate_duplicates
arrange Arrange rows by column values
arrange.default Arrange rows by column values
as_matrix Get matrix from tibble

-- B --

bind Efficiently bind multiple data frames by row and column
bind_cols Efficiently bind multiple data frames by row and column
bind_rows Efficiently bind multiple data frames by row and column
breast_tcga_mini Data set

-- C --

check_if_counts_is_na Check whether there are NA counts
check_if_duplicated_genes Check whether there are duplicated genes/transcripts
check_if_wrong_input Check whether there are NA counts
cluster_elements Get clusters of elements (e.g., samples or transcripts)
cluster_elements-method cluster_elements
cluster_elements-method cluster_elements
cluster_elements-method cluster_elements
cluster_elements-method cluster_elements
cluster_elements-method cluster_elements
counts Example data set
counts_ensembl Counts with ensembl annotation
counts_mini Example data set reduced
create_tt_from_bam_sam_bulk Convert bam/sam files to a tidy gene transcript counts data frame
create_tt_from_tibble_bulk Create tt object from tibble

-- D --

deconvolve_cellularity Get cell type proportions from samples
deconvolve_cellularity-method deconvolve_cellularity
deconvolve_cellularity-method deconvolve_cellularity
deconvolve_cellularity-method deconvolve_cellularity
deconvolve_cellularity-method deconvolve_cellularity
deconvolve_cellularity-method deconvolve_cellularity
distinct distinct
drop_attr Drop attribute to abject
drop_class Remove class to abject

-- E --

ensembl_symbol_mapping Data set
ensembl_to_symbol Add transcript symbol column from ensembl id for human and mouse data
ensembl_to_symbol-method ensembl_to_symbol
ensembl_to_symbol-method ensembl_to_symbol
ensembl_to_symbol-method ensembl_to_symbol
error_if_counts_is_na Check whether there are NA counts
error_if_duplicated_genes Check whether there are duplicated genes/transcripts
error_if_log_transformed Check whether a numeric vector has been log transformed
error_if_wrong_input Check whether there are NA counts

-- F --

fill_NA_using_formula This function is needed for DE in case the matrix is not rectangular, but includes NA
fill_NA_with_row_median This function is needed for DE in case the matrix is not rectangular, but includes NA
filter Subset rows using column values
flybaseIDs flybaseIDs
full_join Full join datasets

-- G --

get_abundance_norm_if_exists Get column names either from user or from attributes
get_adjusted_counts_for_unwanted_variation_bulk Get adjusted count for some batch effect
get_cell_type_proportions Get cell type proportions from cibersort
get_clusters_kmeans_bulk Get K-mean clusters to a tibble
get_clusters_SNN_bulk Get SNN shared nearest neighbour clusters to a tibble
get_differential_transcript_abundance_bulk Get differential transcription information to a tibble using edgeR.
get_elements Get column names either from user or from attributes
get_elements_features Get column names either from user or from attributes
get_elements_features_abundance Get column names either from user or from attributes
get_reduced_dimensions_MDS_bulk Get dimensionality information to a tibble using MDS
get_reduced_dimensions_PCA_bulk Get principal component information to a tibble using PCA
get_reduced_dimensions_TSNE_bulk Get principal component information to a tibble using tSNE
get_rotated_dimensions Get rotated dimensions of two principal components or MDS dimension of choice, of an angle
get_sample Get column names either from user or from attributes
get_sample_counts Get column names either from user or from attributes
get_sample_transcript Get column names either from user or from attributes
get_sample_transcript_counts Get column names either from user or from attributes
get_scaled_counts_bulk Get a tibble with scaled counts using TMM
get_symbol_from_ensembl after wget, this function merges hg37 and hg38 mapping data bases - Do not execute!
get_transcript Get column names either from user or from attributes
get_x_y_annotation_columns get_x_y_annotation_columns
group_by Group by one or more variables

-- I --

ifelse2_pipe This is a generalisation of ifelse that acceots an object and return an objects
ifelse_pipe This is a generalisation of ifelse that acceots an object and return an objects
impute_abundance Impute transcript abundance if missing from sample-transcript pairs
impute_abundance-method impute_abundance
impute_abundance-method impute_abundance
impute_abundance-method impute_abundance
impute_abundance-method impute_abundance
impute_abundance-method impute_abundance
inner_join Inner join datasets

-- K --

keep_abundant Filter abundant transcripts
keep_abundant-method keep_abundant
keep_abundant-method keep_abundant
keep_abundant-method keep_abundant
keep_abundant-method keep_abundant
keep_abundant-method keep_abundant
keep_variable Filter variable transcripts
keep_variable-method keep_variable
keep_variable-method keep_variable
keep_variable-method keep_variable
keep_variable-method keep_variable
keep_variable-method keep_variable
keep_variable_transcripts Identify variable genes for dimensionality reduction

-- L --

left_join Left join datasets

-- M --

mutate Create, modify, and delete columns

-- N --

nest nest

-- P --

parse_formula .formula parser
pivot_sample Extract sampe-wise information
pivot_sample-method pivot_sample
pivot_sample-method pivot_sample
pivot_sample-method pivot_sample
pivot_transcript Extract transcript-wise information
pivot_transcript-method pivot_transcript
pivot_transcript-method pivot_transcript
pivot_transcript-method pivot_transcript
prepend From rlang deprecated

-- R --

reduce_dimensions Dimension reduction of the transcript abundance data
reduce_dimensions-method reduce_dimensions
reduce_dimensions-method reduce_dimensions
reduce_dimensions-method reduce_dimensions
reduce_dimensions-method reduce_dimensions
reduce_dimensions-method reduce_dimensions
remove_redundancy Drop redundant elements (e.g., samples) for which feature (e.g., transcript/gene) aboundances are correlated
remove_redundancy-method remove_redundancy
remove_redundancy-method remove_redundancy
remove_redundancy-method remove_redundancy
remove_redundancy-method remove_redundancy
remove_redundancy-method remove_redundancy
remove_redundancy_elements_though_reduced_dimensions Identifies the closest pairs in a MDS contaxt and return one of them
remove_redundancy_elements_through_correlation Drop redundant elements (e.g., samples) for which feature (e.g., genes) aboundances are correlated
rename Rename columns
right_join Right join datasets
rotate_dimensions Rotate two dimensions (e.g., principal components) of an arbitrary angle
rotate_dimensions-method rotate_dimensions
rotate_dimensions-method rotate_dimensions
rotate_dimensions-method rotate_dimensions
rotate_dimensions-method rotate_dimensions
rotate_dimensions-method rotate_dimensions
rowwise Group input by rows
run_llsr Perform linear equation system analysis through llsr

-- S --

scale_abundance Scale the counts of transcripts/genes
scale_abundance-method scale_abundance
scale_abundance-method scale_abundance
scale_abundance-method scale_abundance
scale_abundance-method scale_abundance
scale_abundance-method scale_abundance
scale_design Scale design matrix
se SummarizedExperiment
select_closest_pairs Sub function of remove_redundancy_elements_though_reduced_dimensions
se_mini SummarizedExperiment mini for vignette
summarise Summarise each group to fewer rows
symbol_to_entrez Get ENTREZ id from gene SYMBOL

-- T --

test_differential_abundance Add differential transcription information to a tbl using edgeR.
test_differential_abundance-method test_differential_abundance
test_differential_abundance-method test_differential_abundance
test_differential_abundance-method test_differential_abundance
test_differential_abundance-method test_differential_abundance
test_differential_abundance-method test_differential_abundance
test_gene_enrichment analyse gene enrichment with EGSEA
test_gene_enrichment-method test_gene_enrichment
test_gene_enrichment-method test_gene_enrichment
test_gene_enrichment-method test_gene_enrichment
test_gene_enrichment_bulk_EGSEA Get gene enrichment analyses using EGSEA
tidybulk Creates a 'tt' object from a 'tbl"
tidybulk-method tidybulk
tidybulk-method tidybulk
tidybulk-method tidybulk
tidybulk-method tidybulk
tidybulk_SAM_BAM Creates a 'tt' object from a list of file names of BAM/SAM
tidybulk_SAM_BAM-method tidybulk_SAM_BAM
tidybulk_to_SummarizedExperiment tidybulk_to_SummarizedExperiment

-- U --

ungroup Group by one or more variables

-- X --

X_cibersort Cibersort reference