Package: CRISPRseek
Type: Package
Title: Design of target-specific guide RNAs in CRISPR-Cas9,
        genome-editing systems
Version: 1.27.6
Date: 2020-04-22
Encoding: UTF-8
Author: Lihua Julie Zhu, Benjamin R. Holmes, Hervé Pagès, Michael Lawrence, Isana Veksler-Lublinsky, Victor Ambros, Neil Aronin and Michael Brodsky
Maintainer: Lihua Julie Zhu <julie.zhu@umassmed.edu>
Depends: R (>= 3.0.1), BiocGenerics, Biostrings
Imports: parallel, data.table, seqinr, S4Vectors (>= 0.9.25), IRanges,
        BSgenome, BiocParallel, hash, methods
Suggests: RUnit, BiocStyle, BSgenome.Hsapiens.UCSC.hg19,
        TxDb.Hsapiens.UCSC.hg19.knownGene, org.Hs.eg.db
Description: The package includes functions to find potential guide RNAs for the CRISPR editing system including Base Editors and the Prime Editor for input target sequences, optionally filter guide RNAs without restriction enzyme cut site, or without paired guide RNAs, genome-wide search for off-targets, score, rank, fetch flank sequence and indicate whether the target and off-targets are located in exon region or not. Potential guide RNAs are annotated with total score of the top5 and topN off-targets, detailed topN mismatch sites, restriction enzyme cut sites, and paired guide RNAs. This package leverages Biostrings and BSgenome packages.
License: GPL (>= 2)
LazyLoad: yes
biocViews: ImmunoOncology, GeneRegulation, SequenceMatching, CRISPR
git_url: https://git.bioconductor.org/packages/CRISPRseek
git_branch: master
git_last_commit: 282fa86
git_last_commit_date: 2020-04-21
Date/Publication: 2020-04-22
NeedsCompilation: no
Packaged: 2020-04-23 00:55:25 UTC; biocbuild
Built: R 4.0.0; ; 2020-04-23 13:16:14 UTC; unix
